BMRB Entry 4376
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4376
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Title: Chemical shift assignments, 3JHNHA coupling constants, secondary structure and 15N{1H} Heteronuclear NOE values of the N-domain of VAT (VCP like ATPase of Thermoplasma). A group II AAA ATPase.
Deposition date: 1999-08-13 Original release date: 1999-10-13
Authors: Coles, Murray; Diercks, Tammo; Liermann, Jens; Groeger, Adriane; Rockel, Beate; Baumeister, Wolfgang; Koretke, Kirstin; Lupas, Andrei; Peters, Juergen; Kessler, Horst
Citation: Coles, Murray; Diercks, Tammo; Liermann, Jens; Groeger, Adriane; Rockel, Beate; Baumeister, Wolfgang; Koretke, Kirstin; Lupas, Andrei; Peters, Juergen; Kessler, Horst. "The Solution Strucutre of VAT-N Reveals a Missing Link in the Evolution of Complex Enzymes from a Simple bbab Element." Current Biology 9, 1158-1168 (1999).
Assembly members:
VCP-like ATPase of Thermoplasma N-domain, polymer, 185 residues, 21702 Da.
Natural source: Common Name: Thermoplasma acidophilum Taxonomy ID: 2302 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Thermoplasma acididophilum
Experimental source: Production method: recombinant technology Host organism: BL21[DE3] Escherichia
Entity Sequences (FASTA):
VCP-like ATPase of Thermoplasma N-domain: MESNNGIILRVAEANSTDPG
MSRVRLDESSRRLLDAEIGD
VVEIEKVRKTVGRVYRARPE
DENKGIVRIDSVMRNNCGAS
IGDKVKVRKVRTEIAKKVTL
APIIRKDQRLKFGEGIEEYV
QRALIRRPMLEQDNISVPGL
TLAGQTGLLFKVVKTLPSKV
PVEIGEETKIEIREEPASEV
LEEGG
- assigned_chemical_shifts
- coupling_constants
- heteronucl_NOEs
Data type | Count |
13C chemical shifts | 785 |
15N chemical shifts | 183 |
1H chemical shifts | 1319 |
coupling constants | 150 |
heteronuclear NOE values | 183 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | VATN | 1 |
Entities:
Entity 1, VATN 185 residues - 21702 Da.
1 | MET | GLU | SER | ASN | ASN | GLY | ILE | ILE | LEU | ARG | ||||
2 | VAL | ALA | GLU | ALA | ASN | SER | THR | ASP | PRO | GLY | ||||
3 | MET | SER | ARG | VAL | ARG | LEU | ASP | GLU | SER | SER | ||||
4 | ARG | ARG | LEU | LEU | ASP | ALA | GLU | ILE | GLY | ASP | ||||
5 | VAL | VAL | GLU | ILE | GLU | LYS | VAL | ARG | LYS | THR | ||||
6 | VAL | GLY | ARG | VAL | TYR | ARG | ALA | ARG | PRO | GLU | ||||
7 | ASP | GLU | ASN | LYS | GLY | ILE | VAL | ARG | ILE | ASP | ||||
8 | SER | VAL | MET | ARG | ASN | ASN | CYS | GLY | ALA | SER | ||||
9 | ILE | GLY | ASP | LYS | VAL | LYS | VAL | ARG | LYS | VAL | ||||
10 | ARG | THR | GLU | ILE | ALA | LYS | LYS | VAL | THR | LEU | ||||
11 | ALA | PRO | ILE | ILE | ARG | LYS | ASP | GLN | ARG | LEU | ||||
12 | LYS | PHE | GLY | GLU | GLY | ILE | GLU | GLU | TYR | VAL | ||||
13 | GLN | ARG | ALA | LEU | ILE | ARG | ARG | PRO | MET | LEU | ||||
14 | GLU | GLN | ASP | ASN | ILE | SER | VAL | PRO | GLY | LEU | ||||
15 | THR | LEU | ALA | GLY | GLN | THR | GLY | LEU | LEU | PHE | ||||
16 | LYS | VAL | VAL | LYS | THR | LEU | PRO | SER | LYS | VAL | ||||
17 | PRO | VAL | GLU | ILE | GLY | GLU | GLU | THR | LYS | ILE | ||||
18 | GLU | ILE | ARG | GLU | GLU | PRO | ALA | SER | GLU | VAL | ||||
19 | LEU | GLU | GLU | GLY | GLY |
Samples:
VATN_sample_N15: VCP-like ATPase of Thermoplasma N-domain, [U-15N], 1.40 mM; phosphate buffer 40 mM; sodium chloride 80 mM; sodium azide 10 mM; D2O 10.0%; H2O 90.0%
VATN_sample_dl: VCP-like ATPase of Thermoplasma N-domain, [U-15N; U-13C], 1.20 mM; phosphate buffer 100 mM; sodium chloride 200 mM; sodium azide 10 mM; D2O 10.0%; H2O 90.0%
sample_conditions_all: pH: 5.9; temperature: 320 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
not available | not available | not available | sample_conditions_all |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 750 MHz
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts