BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 4562

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4562

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Title: 1H and 13C chemical shift assignments of native hen egg white lysozyme

Deposition date: 1999-12-14 Original release date: 2000-01-12

Authors: Wang, Yunjun; Bjorndahl, Trent; Wishart, David

Citation: Wang, Yunjun; Bjorndahl, Trent; Wishart, David. "Letter to the Editor: Complete 1H and Non-carbonylic 13C Assignments of Native Hen Egg-white Lysozyme"  J. Biomol. NMR 17, 83-84 (2000).

Assembly members:
hen egg white lysozyme, polymer, 129 residues, 14296 Da.

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
hen egg white lysozyme: KVFGRCELAAAMKRHGLDNY RGYSLGNWVCAAKFESNFNT QATNRNTDGSTDYGILQINS RWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVS DGNGMNAWVAWRNRCKGTDV QAWIRGCRL

Data sets:
Data typeCount
1H chemical shifts831
13C chemical shifts407

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hen Egg White Lysozyme1

Entities:

Entity 1, Hen Egg White Lysozyme 129 residues - 14296 Da.

1   LYSVALPHEGLYARGCYSGLULEUALAALA
2   ALAMETLYSARGHISGLYLEUASPASNTYR
3   ARGGLYTYRSERLEUGLYASNTRPVALCYS
4   ALAALALYSPHEGLUSERASNPHEASNTHR
5   GLNALATHRASNARGASNTHRASPGLYSER
6   THRASPTYRGLYILELEUGLNILEASNSER
7   ARGTRPTRPCYSASNASPGLYARGTHRPRO
8   GLYSERARGASNLEUCYSASNILEPROCYS
9   SERALALEULEUSERSERASPILETHRALA
10   SERVALASNCYSALALYSLYSILEVALSER
11   ASPGLYASNGLYMETASNALATRPVALALA
12   TRPARGASNARGCYSLYSGLYTHRASPVAL
13   GLNALATRPILEARGGLYCYSARGLEU

Samples:

HEWL_1: hen egg white lysozyme 10 mM

cond_1: pH: 3.6; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D-NOESYHEWL_1not availablenot available
2D-TOCSYHEWL_1not availablenot available
2D 13C HSQC(aliphatic)HEWL_1not availablenot available
2D 13C HSQC(aromatic)HEWL_1not availablenot available
2D 13C HSQCTOCSYHEWL_1not availablenot available

Software:

VNMR v5.1, Varian -

NMR spectrometers:

  • Varian Inova 800 MHz

Related Database Links:

BMRB 1093 1104 1105 11052 1650 1651 1653 1770 1772 1798 1799 1800 1801 1802 1803 18304 18305 1865 1881 1882 1883 1884 1975 1977 2231 2446 2786 2858 2917 2957
PDB
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