BMRB Entry 4751

Name: Assignment of 1H and 15N resonances of mouse lysozyme
Published: 2000-12-18

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4751

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Assignment of 1H and 15N resonances of mouse lysozyme

Deposition date: 2000-06-05 Original release date: 2000-12-18

Authors: Obita, Takayuki; Ueda, Tadashi; Tanaka, Yoshitsugu; Hashimoto, Yoshio; Imoto, Taiji

Citation: Obita, Takayuki; Ueda, Tadashi; Tanaka, Yoshitsugu; Hashimoto, Yoshio; Imoto, Taiji. "Letter to the Editor: Assignment of 1H and 15N resonances of mouse lysozyme M" J. Biomol. NMR 18, 361-362 (2000).

Assembly members:
mouse lysozyme, polymer, 130 residues, 14820 Da.

Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris

Entity Sequences (FASTA):
mouse lysozyme: KVYERCEFARTLKRNGMAGY YGVSLADWVCLAQHESNYNT RATNYNRGDQSTDYGIFQIN SRYWCNDGKTPRAVNACGIN CSALLQDDITAAIQCAKRVV RDPQGIRAWVAWRAHCQNRD LSQYIRNCGV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	127
1H chemical shifts	709

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mouse lysozyme	1

Entities:

Entity 1, mouse lysozyme 130 residues - 14820 Da.

1	LYS	VAL	TYR	GLU	ARG	CYS	GLU	PHE	ALA	ARG
2	THR	LEU	LYS	ARG	ASN	GLY	MET	ALA	GLY	TYR
3	TYR	GLY	VAL	SER	LEU	ALA	ASP	TRP	VAL	CYS
4	LEU	ALA	GLN	HIS	GLU	SER	ASN	TYR	ASN	THR
5	ARG	ALA	THR	ASN	TYR	ASN	ARG	GLY	ASP	GLN
6	SER	THR	ASP	TYR	GLY	ILE	PHE	GLN	ILE	ASN
7	SER	ARG	TYR	TRP	CYS	ASN	ASP	GLY	LYS	THR
8	PRO	ARG	ALA	VAL	ASN	ALA	CYS	GLY	ILE	ASN
9	CYS	SER	ALA	LEU	LEU	GLN	ASP	ASP	ILE	THR
10	ALA	ALA	ILE	GLN	CYS	ALA	LYS	ARG	VAL	VAL
11	ARG	ASP	PRO	GLN	GLY	ILE	ARG	ALA	TRP	VAL
12	ALA	TRP	ARG	ALA	HIS	CYS	GLN	ASN	ARG	ASP
13	LEU	SER	GLN	TYR	ILE	ARG	ASN	CYS	GLY	VAL

Samples:

sample_1: mouse lysozyme, [U-15N], 1.0 mM

sample_conditions_1: pH: 3.8; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
15N-edited NOESY	not available	not available	not available
15N-edited TOCSY	not available	not available	not available
1H NOESY	not available	not available	not available
1H TOCSY	not available	not available	not available
1H COSY	not available	not available	not available

Software:

NMR-PIPE - raw spectral data transformations

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1IVM
DBJ	BAE28595 BAE30022 BAE31835 BAE32025 BAE34954
GenBank	AAA39473 AAH02069 AAH54463 EDL21835
REF	NP_059068
SWISS-PROT	P08905

Biological Magnetic Resonance Data Bank