BMRB Entry 4831

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Lysozyme
Published: 2001-05-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4831

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Lysozyme PubMed: 11274458

Deposition date: 2000-09-15 Original release date: 2001-05-01

Authors: Schwalbe, Harald; Grimshaw, Shaun; Spencer, Andrew; Buck, Matthias; Boyd, Jonathan; Dobson, Christopher; Redfield, Christina; Smith, Lorna

Citation: Schwalbe, Harald; Grimshaw, Shaun; Spencer, Andrew; Buck, Matthias; Boyd, Jonathan; Dobson, Christopher; Redfield, Christina; Smith, Lorna. "A Refined Solution Structure of Hen Lysozyme Determined Using Residual Dipolar Coupling Data" Protein Sci. 10, 677-688 (2001).

Assembly members:
hen egg white lysozyme, polymer, 129 residues, Formula weight is not available

Natural source: Common Name: hen Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
hen egg white lysozyme: KVFGRCELAAAMKRHGLDNY RGYSLGNWVCAAKFESNFNT EATNRNTDGSTDYGILQINS RWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVS DGNGMNAWVAWRNRCKGTDV QAWIRGCRL

Data sets:

assigned_chemical_shifts
- shift_set_1
RDCs
- residual_dipolar_couplings_1
- residual_dipolar_couplings_2

Data type	Count
13C chemical shifts	364
15N chemical shifts	147
residual dipolar couplings	209

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	lysozyme	1

Entities:

Entity 1, lysozyme 129 residues - Formula weight is not available

1

LYS

VAL

PHE

GLY

ARG

CYS

GLU

LEU

ALA

2

ALA

MET

LYS

ARG

HIS

GLY

LEU

ASP

ASN

TYR

3

ARG

GLY

TYR

SER

LEU

GLY

ASN

TRP

VAL

CYS

4

ALA

LYS

PHE

GLU

SER

ASN

PHE

ASN

THR

5

GLU

ALA

THR

ASN

ARG

ASN

THR

ASP

GLY

SER

6

THR

ASP

TYR

GLY

ILE

LEU

GLN

ILE

ASN

SER

7

ARG

TRP

CYS

ASN

ASP

GLY

ARG

THR

PRO

8

GLY

SER

ARG

ASN

LEU

CYS

ASN

ILE

PRO

CYS

9

SER

ALA

LEU

SER

ASP

ILE

THR

ALA

10

SER

VAL

ASN

CYS

ALA

LYS

ILE

VAL

SER

11

ASP

GLY

ASN

GLY

MET

ASN

ALA

TRP

VAL

ALA

12

TRP

ARG

ASN

ARG

CYS

LYS

GLY

THR

ASP

VAL

13

GLN

ALA

TRP

ILE

ARG

GLY

CYS

ARG

LEU

Samples:

sample_1: hen egg white lysozyme, [U-95% 13C; U-98% 15N], 1.5 mM

sample_2: hen egg white lysozyme, [U-15N], 0.5 mM; DMPC/DHPC 5%; phosphate 10 mM; H2O 93%; D2O 7%

sample_3: hen egg white lysozyme, [U-15N], 0.5 mM; DMPC/DHPC/CTAB 7.5%; phosphate 10 mM; H2O 93%; D2O 7%

ex-cond_1: pH: 3.8; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY-HSQC	not available	not available	ex-cond_1
1H-15N TOCSY-HSQC	not available	not available	ex-cond_1
1H-15N HSQC-NOESY-HSQC	not available	not available	ex-cond_1
HNCO	not available	not available	ex-cond_1
HNCA	not available	not available	ex-cond_1
CBCACONH	not available	not available	ex-cond_1
CBCANH	not available	not available	ex-cond_1
HCCH-TOCSY	not available	not available	ex-cond_1
HCCH-NOESY	not available	not available	ex-cond_1
SOFT HCCH-COSY	not available	not available	ex-cond_1
SOFT HCCH-E.COSY	not available	not available	ex-cond_1
HMQCJ	not available	not available	ex-cond_1

Software:

FELIX v98 - Assignment

NMR spectrometers:

Omega . 750 MHz
Omega . 600 MHz
Omega . 500 MHz
Bruker . 800 MHz
Bruker . 600 MHz