BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4865

Title: Backbone 1H, 13C, 15N Chemical shift Assignment for OMTKY3 bound to bovine Chymotrypsin Aa

Authors: Song, Jikui; Markley, John

Citation: Song, Jikui; Markley, John. "NMR Chemical shift mapping of the binding site of a protein proteinase inhibitor: changes in the 1H, 13C and 15N NMR chemical shifts of turkey ovomucoid third domain upon binding to bovine chymotrypsin Aa"  J. Mol. Recognit. 14, 166-171 (2001).

Assembly members:
Ovomucoid Third Domain from Turkey, polymer, 56 residues, Formula weight is not available
bovine chymotrypsin Aa, polymer, 241 residues, Formula weight is not available

Natural source:   Common Name: Turkey   Taxonomy ID: 9103   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meleagris Gallopavo

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Ovomucoid Third Domain from Turkey: LAAVSVDCSEYPKPACTLEY RPLCGSDNKTYGNKCNFCNA VVESNGTLTLSHFGKC
bovine chymotrypsin Aa: CGVPAIQPVLSGLIVNGEEA VPGSWPWQVSLQDKTGFHFC GGSLINENWVVTAAHCGVTT SDVVVAGEFDQGSSSEKIQK LKIAKVFKNSKYNSLTINND ITLLKLSTAASFSQTVSAVC LPSASDDFAAGTTCVTTGWG LTRYANTPDRLQQASLPLLS NTNCKKYWGTKIKDAMICAG ASGVSSCMGDSGGPLVCKKN GAWTLVGIVSWGSSTCSTST PGVYARVTALVNWVQQTLAA N

Data sets:
Data typeCount
1H chemical shifts45
13C chemical shifts85
15N chemical shifts46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OMTKY31
2bovine chymotrypsin Aa2

Entities:

Entity 1, OMTKY3 56 residues - Formula weight is not available

1   LEUALAALAVALSERVALASPCYSSERGLU
2   TYRPROLYSPROALACYSTHRLEUGLUTYR
3   ARGPROLEUCYSGLYSERASPASNLYSTHR
4   TYRGLYASNLYSCYSASNPHECYSASNALA
5   VALVALGLUSERASNGLYTHRLEUTHRLEU
6   SERHISPHEGLYLYSCYS

Entity 2, bovine chymotrypsin Aa 241 residues - Formula weight is not available

1   CYSGLYVALPROALAILEGLNPROVALLEU
2   SERGLYLEUILEVALASNGLYGLUGLUALA
3   VALPROGLYSERTRPPROTRPGLNVALSER
4   LEUGLNASPLYSTHRGLYPHEHISPHECYS
5   GLYGLYSERLEUILEASNGLUASNTRPVAL
6   VALTHRALAALAHISCYSGLYVALTHRTHR
7   SERASPVALVALVALALAGLYGLUPHEASP
8   GLNGLYSERSERSERGLULYSILEGLNLYS
9   LEULYSILEALALYSVALPHELYSASNSER
10   LYSTYRASNSERLEUTHRILEASNASNASP
11   ILETHRLEULEULYSLEUSERTHRALAALA
12   SERPHESERGLNTHRVALSERALAVALCYS
13   LEUPROSERALASERASPASPPHEALAALA
14   GLYTHRTHRCYSVALTHRTHRGLYTRPGLY
15   LEUTHRARGTYRALAASNTHRPROASPARG
16   LEUGLNGLNALASERLEUPROLEULEUSER
17   ASNTHRASNCYSLYSLYSTYRTRPGLYTHR
18   LYSILELYSASPALAMETILECYSALAGLY
19   ALASERGLYVALSERSERCYSMETGLYASP
20   SERGLYGLYPROLEUVALCYSLYSLYSASN
21   GLYALATRPTHRLEUVALGLYILEVALSER
22   TRPGLYSERSERTHRCYSSERTHRSERTHR
23   PROGLYVALTYRALAARGVALTHRALALEU
24   VALASNTRPVALGLNGLNTHRLEUALAALA
25   ASN

Samples:

Sample_1: Ovomucoid Third Domain from Turkey, [U-13C; U-15N], 2 mM; bovine chymotrypsin Aa 2 mM

condition_1: pH: 7.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 6881 960 961 962 963 964 965 966 967 967 965 963 961 6881
PDB
REF XP_003583409 XP_003587247 XP_608091
SP P00766
SWISS-PROT P00766