BMRB Entry 4876
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4876
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Title: 1H,13C,15N assignment of Ca2+-bound state of Canine Milk Lysozyme at 30deg
Deposition date: 2000-10-24 Original release date: 2001-04-30
Authors: Kobashigawa, Yoshihiro; Nitta, Katsutoshi; Tsuda, Sakae
Citation: Kobashigawa, Yoshihiro; Miura, Kazunori; Demura, Makoto; Nemoto, Nobuaki; Koshiba, Takumi; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignment of 1H, 13C, and 15N Resonances of Canine Milk Lysozyme" J. Biomol. NMR 19, 387-388 (2001).
Assembly members:
canine milk lysozyme, polymer, 130 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
canine milk lysozyme: SKIFSKCELARKLKSMGMDG
FHGYSLANWVCMAEYESNFN
TQAFNGRNSNGSSDYGIFQL
NSKWWCKSNSHSSANACNIM
CSKFLDDNIDDDIACAKRVV
KDPNGMSAWVAWVKHCKGKD
LSKYLASCNL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 244 |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 260 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | canine milk lysozyme | 1 |
| 2 | Ca | 2 |
Entities:
Entity 1, canine milk lysozyme 130 residues - Formula weight is not available
| 1 | SER | LYS | ILE | PHE | SER | LYS | CYS | GLU | LEU | ALA | |
| 2 | ARG | LYS | LEU | LYS | SER | MET | GLY | MET | ASP | GLY | |
| 3 | PHE | HIS | GLY | TYR | SER | LEU | ALA | ASN | TRP | VAL | |
| 4 | CYS | MET | ALA | GLU | TYR | GLU | SER | ASN | PHE | ASN | |
| 5 | THR | GLN | ALA | PHE | ASN | GLY | ARG | ASN | SER | ASN | |
| 6 | GLY | SER | SER | ASP | TYR | GLY | ILE | PHE | GLN | LEU | |
| 7 | ASN | SER | LYS | TRP | TRP | CYS | LYS | SER | ASN | SER | |
| 8 | HIS | SER | SER | ALA | ASN | ALA | CYS | ASN | ILE | MET | |
| 9 | CYS | SER | LYS | PHE | LEU | ASP | ASP | ASN | ILE | ASP | |
| 10 | ASP | ASP | ILE | ALA | CYS | ALA | LYS | ARG | VAL | VAL | |
| 11 | LYS | ASP | PRO | ASN | GLY | MET | SER | ALA | TRP | VAL | |
| 12 | ALA | TRP | VAL | LYS | HIS | CYS | LYS | GLY | LYS | ASP | |
| 13 | LEU | SER | LYS | TYR | LEU | ALA | SER | CYS | ASN | LEU |
Entity 2, Ca - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample1: canine milk lysozyme, [U-15N; U-13C], 1.0 mM
Ex-cond_1: pH: 4.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| experiment_1 | sample1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 500 MHz
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SPARKY: Backbone
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