BMRB Entry 4943
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4943
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Title: 1H Chemical shift Assignments for Lysozymes marketed by Seikagaku and Sigma companies at pH 3.8, 308K
Deposition date: 2001-01-19 Original release date: 2003-05-21
Authors: Poznanski, Jaroslaw; Georgalis, Yannis; Wehr, Lorin; Zielenkiewicz, Piotr; Saenger, Wolfram
Citation: Poznanski, Jaroslaw; Georgalis, Yannis; Wehr, Lorin; Zielenkiewicz, Piotr; Saenger, Wolfram. "Comparison of Two Different Lysozyme Types Under Native and Crystallization Conditions using Two-Dimensional NMR and Dynamic Light Scattering " Biophys. Chem. ., .-. (2002).
Assembly members:
Chicken Egg White Lysozyme, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: vendor
Entity Sequences (FASTA):
Chicken Egg White Lysozyme: KVFGRCELAAAMKRHGLDNY
RGYSLGNWVCAAKFESNFNT
QATNRNTDGSTDYGILQINS
RWWCNDGRTPGSRNLCNIPC
SALLSSDITASVNCAKKIVS
DGNGMNAWVAWRNRCKGTDV
QAWIRGCRLDRSR
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | lysozyme | 1 |
Entities:
Entity 1, lysozyme 133 residues - Formula weight is not available
| 1 | LYS | VAL | PHE | GLY | ARG | CYS | GLU | LEU | ALA | ALA | ||||
| 2 | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | TYR | ||||
| 3 | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | CYS | ||||
| 4 | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | THR | ||||
| 5 | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | SER | ||||
| 6 | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | SER | ||||
| 7 | ARG | TRP | TRP | CYS | ASN | ASP | GLY | ARG | THR | PRO | ||||
| 8 | GLY | SER | ARG | ASN | LEU | CYS | ASN | ILE | PRO | CYS | ||||
| 9 | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | ALA | ||||
| 10 | SER | VAL | ASN | CYS | ALA | LYS | LYS | ILE | VAL | SER | ||||
| 11 | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | ALA | ||||
| 12 | TRP | ARG | ASN | ARG | CYS | LYS | GLY | THR | ASP | VAL | ||||
| 13 | GLN | ALA | TRP | ILE | ARG | GLY | CYS | ARG | LEU | ASP | ||||
| 14 | ARG | SER | ARG |
Samples:
sample_A: Chicken Egg White Lysozyme 10 mM
sample_B: Chicken Egg White Lysozyme 10 mM
cond: pH: 3.8; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | not available | not available | cond |
| 1H-1H NOESY | not available | not available | cond |
Software:
nmrpipe v1.7 - data processing
XEasy v1.3.11 - peak assignment, sequential assignment
NMR spectrometers:
- Varian Unity Plus 500 MHz
Related Database Links:
| BMRB | 1093 1104 1105 11052 1650 1651 1653 1770 1772 1798 1799 1800 1801 1802 1803 18304 18305 1865 1881 1882 1883 1884 1975 1977 2231 2446 2786 2858 2917 2957 4562 4831 5068 5069 568 569 5803 5804 6415 7143 7144 7159 7160 791 |
| PDB | |
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