BMRB Entry 4970

Assembly:

Entities:

Entity 1, Calmodulin 148 residues - Formula weight is not available

Entity 2, smooth muscle myosin light chain kinase 21 residues - Formula weight is not available

Samples:

sample_one: Calmodulin, [U-13C; U-15N], 1.2 mM; smooth muscle myosin light chain kinase calmodulin binding domain 2.4 mM; imidazole, [U-2H], 10 – 20 mM; KCl 100 mM; CaCl2 6 mM; NaN3 0.02%; H2O 93%; D2O 7%

sample_two: Calmodulin, [U-50% 2H; U-13C; U-15N], 1.2 mM; smooth muscle myosin light chain kinase calmodulin binding domain 2.4 mM; bis-tris, [U-2H], 10 – 20 mM; KCl 100 mM; CaCl2 6 mM; NaN3 0.02%; H2O 93%; D2O 7%

sample_conditions_1: pH: 5.0; temperature: 295 K

sample_conditions_2: pH: 5.0; temperature: 308 K

sample_conditions_3: pH: 5.0; temperature: 320 K

sample_conditions_4: pH: 5.0; temperature: 333 K

sample_conditions_5: pH: 5.0; temperature: 346 K

sample_conditions_6: pH: 5.0; temperature: 288 K

sample_conditions_7: pH: 5.0; temperature: 301 K

sample_conditions_8: pH: 5.0; temperature: 311 K

sample_conditions_9: pH: 5.0; temperature: 314 K

sample_conditions_10: pH: 5.0; temperature: 317 K

sample_conditions_11: pH: 5.0; temperature: 323 K

sample_conditions_12: pH: 5.0; temperature: 326 K

sample_conditions_13: pH: 5.0; temperature: 340 K

Experiments:

Software:

No software information available

NMR spectrometers:

• Varian Inova 600 MHz
• Varian Inova 750 MHz