BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5030

Title: 1H, 13C and 15N Chemical Shift Assignment of the Honeybee Odorant-binding Protein ASP2   PubMed: 11727984

Authors: Lescop, Ewen; Briand, Loic; Pernollet, Jean-Claude; van Heijenoort, Carine; Guittet, Eric

Citation: Lescop, Ewen; Briand, Loic; Pernollet, Jean-Claude; van Heijenoort, Carine; Guittet, Eric. "Letter to the Editor: 1H, 13C and 15N Chemical Shift Assignment of the Honeybee Odorant-binding Protein ASP2"  J. Biomol. NMR 21, 181-182 (2001).

Assembly members:
ASP2, polymer, 123 residues, 13695 Da.

Natural source:   Common Name: Honeybee   Taxonomy ID: 7460   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Apis mellifera

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
ASP2: IDQDTVVAKYMEYLMPDIMP CADELHISEDIATNIQAAKN GADMSQLGCLKACVMKRIEM LKGTELYVEPVYKMIEVVHA GNADDIQLVKGIANECIENA KGETDECNIGNKYTDCYIEK LFS

Data sets:
Data typeCount
13C chemical shifts361
15N chemical shifts119
1H chemical shifts466

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ASP21

Entities:

Entity 1, ASP2 123 residues - 13695 Da.

1   ILEASPGLNASPTHRVALVALALALYSTYR
2   METGLUTYRLEUMETPROASPILEMETPRO
3   CYSALAASPGLULEUHISILESERGLUASP
4   ILEALATHRASNILEGLNALAALALYSASN
5   GLYALAASPMETSERGLNLEUGLYCYSLEU
6   LYSALACYSVALMETLYSARGILEGLUMET
7   LEULYSGLYTHRGLULEUTYRVALGLUPRO
8   VALTYRLYSMETILEGLUVALVALHISALA
9   GLYASNALAASPASPILEGLNLEUVALLYS
10   GLYILEALAASNGLUCYSILEGLUASNALA
11   LYSGLYGLUTHRASPGLUCYSASNILEGLY
12   ASNLYSTYRTHRASPCYSTYRILEGLULYS
13   LEUPHESER

Samples:

15N13C_H2O: ASP2, [U-98% 13C; U-98% 15N], 0.7 – 1.5 mM; TSP 0.1 mM; phosphate buffer 100 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

15N13C_D2O: ASP2, [U-98% 13C; U-98% 15N], 1.3 mM; TSP 0.1 mM; phosphate buffer 100 mM; NaN3 0.1 mM; D2O 100%

15N_H2O: ASP2, [U-98% 15N], 1 mM; TSP 0.1 mM; phosphate buffer 100 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

Cond_D2O: ionic strength: 0.11 M; pH: 7.7; temperature: 308 K

Cond_H2O: ionic strength: 0.11 M; pH: 5.7; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
NOESY-HSQCnot availablenot availablenot available

Software:

XWINNMR v2.6 - acquisition, processing

AURELIA v2.8.4 - Analysis

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
GenBank AAD51945 AAL60418 ABD97844
REF NP_001011591