BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 5068

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5068

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Title: Recombinant hen lysozyme containing the extra N-terminal Met as the standard reference for the study of hen lysozyme variants   PubMed: 11841203

Deposition date: 2001-07-04 Original release date: 2003-12-22

Authors: Noda, Yasuo; Yokota, Atsushi; Horii, Daisuke; Tominaga, Takeshi; Tanisaka, Yoshiaki; Tachibana, Hideki; Segawa, Shin-ichi

Citation: Noda, Yasuo; Yokota, Atsushi; Horii, Daisuke; Tominaga, Takeshi; Tanisaka, Yoshiaki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR Structural Study of Two-disulfide Variant of hen Lysozyme: 2SS[6-127, 30-115]--A Disulfide Intermediate with a Partly Unfolded Structure"  Biochemistry 41, 2130-2139 (2002).

Assembly members:
hen egg white lysozyme, polymer, 130 residues, 14436 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hen egg white lysozyme: MKVFGRCELAAAMKRHGLDN YRGYSLGNWVCAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLCNIP CSALLSSDITASVNCAKKIV SDGNGMNAWVAWRNRCKGTD VQAWIRGCRL

Data sets:
Data typeCount
1H chemical shifts788

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hen lysozyme1

Entities:

Entity 1, hen lysozyme 130 residues - 14436 Da.

1   METLYSVALPHEGLYARGCYSGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   CYSALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPCYSASNASPGLYARGTHR
8   PROGLYSERARGASNLEUCYSASNILEPRO
9   CYSSERALALEULEUSERSERASPILETHR
10   ALASERVALASNCYSALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGCYSLYSGLYTHRASP
13   VALGLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: hen egg white lysozyme, [U-15N], 1.0 – 1.2 mM

EX-cond_1: pH: 3.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
HOHAHAnot availablenot availablenot available
15N 3D-NOESY-HSQCnot availablenot availablenot available
15N 3D-TOCSY-HSQCnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

xwinnmr v2.6 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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PDB
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