BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5075

Title: Solution Structure of Human Apolipoprotein(a) Kringle IV type 6   PubMed: 11781107

Deposition date: 2001-07-09 Original release date: 2002-02-13

Authors: Maderegger, B.; Bermel, W.; Hrzenjak, A.; Kostner, G.; Sterk, H.

Citation: Maderegger, B.; Bermel, W.; Hrzenjak, A.; Kostner, G.; Sterk, H.. "Solution Structure of Human Apolipoprotein(a) Kringle IV type 6"  Biochemistry 41, 660-668 (2002).

Assembly members:
Apolipoprotein(a) kringle IV type6, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Apolipoprotein(a) kringle IV type6: ARIHHHHHHIEGRAPTEQSP GVQDCYHGDGQSYRGSFSTT VTGRTCQSWSSMTPHWHQRT TEYYPNGGLTRNYCRNPDAE ISPWCYTMDPNVRWEYCNLT QCPVTESSVLATSTAVSEQ

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts110
1H chemical shifts612

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Apolipoprotein(a)kringle IV type 61

Entities:

Entity 1, Apolipoprotein(a)kringle IV type 6 119 residues - Formula weight is not available

1   ALAARGILEHISHISHISHISHISHISILE
2   GLUGLYARGALAPROTHRGLUGLNSERPRO
3   GLYVALGLNASPCYSTYRHISGLYASPGLY
4   GLNSERTYRARGGLYSERPHESERTHRTHR
5   VALTHRGLYARGTHRCYSGLNSERTRPSER
6   SERMETTHRPROHISTRPHISGLNARGTHR
7   THRGLUTYRTYRPROASNGLYGLYLEUTHR
8   ARGASNTYRCYSARGASNPROASPALAGLU
9   ILESERPROTRPCYSTYRTHRMETASPPRO
10   ASNVALARGTRPGLUTYRCYSASNLEUTHR
11   GLNCYSPROVALTHRGLUSERSERVALLEU
12   ALATHRSERTHRALAVALSERGLUGLN

Samples:

sample_1: Apolipoprotein(a) kringle IV type6, [U-15N], 0.7 mM; phosphate buffer 15 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_2: Apolipoprotein(a) kringle IV type6, [U-15N; U-13C], 0.7 mM; phosphate buffer 15 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 65 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

NMRPipe v1.6 - processing

ANSIG v3.3 - data analysis

XWINNMR v2.6 - collection

X-PLOR v3.0 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GenBank AAF03677

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts