BMRB Entry 5142
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5142
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Title: Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 4 C PubMed: 11883780
Deposition date: 2001-09-06 Original release date: 2002-05-06
Authors: Kumeta, Hiroyuki; Kobashigawa, Yoshihiro; Miura, Kazunori; Nishimiya, Yoshiyuki; Oka, Chitoshi; Nemoto, Nobuaki; Miura, Ai; Nitta, Katsutoshi; Tsuda, Sakae
Citation: Kumeta, Hiroyuki; Kobashigawa, Yoshihiro; Miura, Kazunori; Nishimiya, Yoshiyuki; Oka, Chitoshi; Nemoto, Nobuaki; Miura, Ai; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignments of 1H, 13C and 15N Resonances of Human Lysozyme at 4 degrees C" J. Biomol. NMR 22, 183-184 (2002).
Assembly members:
human lysozyme, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human lysozyme: KVFERCELARTLKRLGMDGY
RGISLANWMCLAKWESGYNT
RATNYNAGDRSTDYGIFQIN
SRYWCNDGKTPGAVNACHLS
CSALLQDNIADAVACAKRVV
RDPQGIRAWVAWRNRCQNRD
VRQYVQGCGV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 374 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 708 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human lysozyme | 1 |
Entities:
Entity 1, human lysozyme 130 residues - Formula weight is not available
| 1 | LYS | VAL | PHE | GLU | ARG | CYS | GLU | LEU | ALA | ARG | |
| 2 | THR | LEU | LYS | ARG | LEU | GLY | MET | ASP | GLY | TYR | |
| 3 | ARG | GLY | ILE | SER | LEU | ALA | ASN | TRP | MET | CYS | |
| 4 | LEU | ALA | LYS | TRP | GLU | SER | GLY | TYR | ASN | THR | |
| 5 | ARG | ALA | THR | ASN | TYR | ASN | ALA | GLY | ASP | ARG | |
| 6 | SER | THR | ASP | TYR | GLY | ILE | PHE | GLN | ILE | ASN | |
| 7 | SER | ARG | TYR | TRP | CYS | ASN | ASP | GLY | LYS | THR | |
| 8 | PRO | GLY | ALA | VAL | ASN | ALA | CYS | HIS | LEU | SER | |
| 9 | CYS | SER | ALA | LEU | LEU | GLN | ASP | ASN | ILE | ALA | |
| 10 | ASP | ALA | VAL | ALA | CYS | ALA | LYS | ARG | VAL | VAL | |
| 11 | ARG | ASP | PRO | GLN | GLY | ILE | ARG | ALA | TRP | VAL | |
| 12 | ALA | TRP | ARG | ASN | ARG | CYS | GLN | ASN | ARG | ASP | |
| 13 | VAL | ARG | GLN | TYR | VAL | GLN | GLY | CYS | GLY | VAL |
Samples:
sample_1: human lysozyme 1.0 mM
HL04-cond_1: pH: 3.8; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-edited NOESY | not available | not available | not available |
| 15N-edited TOCSY | not available | not available | not available |
| CBCA(CO)NNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| 13C-edited NOESY | not available | not available | not available |
| 1H-13C-1H HCCH-TOCSY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Unity 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts