BMRB Entry 5300
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5300
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Resonance Assignments for the 20.8 KDa C.elegans gene product ORF C32E8.3. Northeast Structural Genomics Consortium Target WR33.
Deposition date: 2002-02-22 Original release date: 2002-06-27
Authors: Monleon, Daniel; YiWen, Chiang; Aramini, James; Swapna, G.V.T.; Kim, Seho; Szyperski, Thomas; Montelione, Gaetano
Citation: Monleon, Daniel; YiWen, Chiang; Aramini, James; Swapna, G.V.T.; Macapagal, Daphne; Gunsalus, Kristin; Kim, Seho; Szyperski, Thomas; Montelione, Gaetano. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the 21 kDa Caenorhabditis elegans homologue of 'brain-specific' protein" J. Biomol. NMR 28, 91-92 (2004).
Assembly members:
ORF C32E8.3, polymer, 190 residues, 20800 Da.
Natural source: Common Name: C.elegans. Taxonomy ID: 6239 Superkingdom: Eubacteria Kingdom: not available Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ORF C32E8.3: MGHHHHHHSHMAAAAGFNWD
DADVKKRWDAFTKFGAATAT
EMTGKNFDKWLKDAGVLDNK
AITGTMTGIAFSKVTGPKKK
ATFDETKKVLAFVAEDRARQ
SKKPIQDELDAITEKLAKLE
APSVGGAAKANAAGVYSRLT
DHTKYTGAHKERFDAEGKGK
GKSGRADTTENTGYVGAYKN
KDSYDKTHGK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 331 |
| 13C chemical shifts | 496 |
| 15N chemical shifts | 175 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C.elegans ORF C32E8.3 | 1 |
Entities:
Entity 1, C.elegans ORF C32E8.3 190 residues - 20800 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | SER | HIS | |
| 2 | MET | ALA | ALA | ALA | ALA | GLY | PHE | ASN | TRP | ASP | |
| 3 | ASP | ALA | ASP | VAL | LYS | LYS | ARG | TRP | ASP | ALA | |
| 4 | PHE | THR | LYS | PHE | GLY | ALA | ALA | THR | ALA | THR | |
| 5 | GLU | MET | THR | GLY | LYS | ASN | PHE | ASP | LYS | TRP | |
| 6 | LEU | LYS | ASP | ALA | GLY | VAL | LEU | ASP | ASN | LYS | |
| 7 | ALA | ILE | THR | GLY | THR | MET | THR | GLY | ILE | ALA | |
| 8 | PHE | SER | LYS | VAL | THR | GLY | PRO | LYS | LYS | LYS | |
| 9 | ALA | THR | PHE | ASP | GLU | THR | LYS | LYS | VAL | LEU | |
| 10 | ALA | PHE | VAL | ALA | GLU | ASP | ARG | ALA | ARG | GLN | |
| 11 | SER | LYS | LYS | PRO | ILE | GLN | ASP | GLU | LEU | ASP | |
| 12 | ALA | ILE | THR | GLU | LYS | LEU | ALA | LYS | LEU | GLU | |
| 13 | ALA | PRO | SER | VAL | GLY | GLY | ALA | ALA | LYS | ALA | |
| 14 | ASN | ALA | ALA | GLY | VAL | TYR | SER | ARG | LEU | THR | |
| 15 | ASP | HIS | THR | LYS | TYR | THR | GLY | ALA | HIS | LYS | |
| 16 | GLU | ARG | PHE | ASP | ALA | GLU | GLY | LYS | GLY | LYS | |
| 17 | GLY | LYS | SER | GLY | ARG | ALA | ASP | THR | THR | GLU | |
| 18 | ASN | THR | GLY | TYR | VAL | GLY | ALA | TYR | LYS | ASN | |
| 19 | LYS | ASP | SER | TYR | ASP | LYS | THR | HIS | GLY | LYS |
Samples:
sample_1: ORF C32E8.3, [U-13C; U-15N], 1.4 mM
sample_2: ORF C32E8.3, [U-13C; U-15N], 0.5 mM
sample_3: ORF C32E8.3, [U-13C; U-15N], 0.5 mM; DMPC:DHCP:CTAB(30:10:1) 4.5%
condition_1: pH: 6.50; temperature: 298 K
condition_2: pH: 6.50; temperature: 298 K
condition_3: pH: 6.50; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| (HA)CA(CO)NH | not available | not available | not available |
| HA(CA)(CO)NH | not available | not available | not available |
| (HA)CANH | not available | not available | not available |
| HA(CA)NH | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
| 13C-edited NOESY | not available | not available | not available |
| 15N-edited NOESY | not available | not available | not available |
| RD-HNCA,HA | not available | not available | not available |
| RD-HA,CA(CO)NH | not available | not available | not available |
| RDC-JHC COUPLED (HA)CANH | not available | not available | not available |
Software:
NMRPipe v2000 - data processing
SPARKY v3.91 - peak picking
AUTOASSIGN v1.8.0 - automated analysis of backbone 1H, 13C and 15N assignments
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
- Varian INOVA 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts