BMRB Entry 5480
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5480
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Title: 1H,15N and 13C assigned Chemical Shifts for a complex of calmodulin with a peptide of the olfactory CNGC channel PubMed: 12885399
Deposition date: 2002-07-25 Original release date: 2003-12-08
Authors: Orsale, Maria; Melino, Sonia; Torre, Vincent; Motta, Andrea; Paci, Maurizio; Desideri, Alessandro; Cicero, Daniel
Citation: Orsale, Maria; Melino, Sonia; Contessa, G.; Torre, Vincent; Andreotti, G.; Motta, Andrea; Paci, Maurizio; Desideri, Alessandro; Cicero, Daniel. "Two distinct calcium-calmodulin interactions with N-terminal regions of the olfactory and rod cyclic nucleotide-gated channels characterized by NMR spectroscopy" FEBS Lett. 548, 11-16 (2003).
Assembly members:
Calmodulin, polymer, 148 residues, 16700 Da.
olfactory channel peptide, polymer, 26 residues, 2900 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus laevis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
olfactory channel peptide: QQRRGGFRRIARLVGVLREW
AYRNFR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1141 |
| 13C chemical shifts | 664 |
| 15N chemical shifts | 157 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin | 1 |
| 2 | olfactory channel peptide | 2 |
| 3 | Calcium ion, I | 3 |
| 4 | Calcium ion, II | 3 |
| 5 | Calcium ion, III | 3 |
| 6 | Calcium ion, IV | 3 |
Entities:
Entity 1, calmodulin 148 residues - 16700 Da.
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, olfactory channel peptide 26 residues - 2900 Da.
| 1 | GLN | GLN | ARG | ARG | GLY | GLY | PHE | ARG | ARG | ILE | ||||
| 2 | ALA | ARG | LEU | VAL | GLY | VAL | LEU | ARG | GLU | TRP | ||||
| 3 | ALA | TYR | ARG | ASN | PHE | ARG |
Entity 3, Calcium ion, I - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Calmodulin, [U-95% 13C; U-95% 15N], 1.2 mM; olfactory channel peptide 1.2 mM; KCl 140 mM; CaCl2 7 mM
sample_2: Calmodulin, [U-15N], 0.4 mM; olfactory channel peptide 0.4 mM; KCl 140 mM; CaCl2 7 mM
Exp-cond_1: pH: 6.7; temperature: 308 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | Exp-cond_1 |
| 15N edited TOCSY (40ms) | not available | not available | Exp-cond_1 |
| 15N edited NOESY (80ms) | not available | not available | Exp-cond_1 |
| HNHA | not available | not available | Exp-cond_1 |
| 1H-15N NOE | not available | not available | Exp-cond_1 |
| HNCA | not available | not available | Exp-cond_1 |
| HN(CO)CA | not available | not available | Exp-cond_1 |
| HNCO | not available | not available | Exp-cond_1 |
| CBCA(CO)NH | not available | not available | Exp-cond_1 |
| HBHA(CO)NH | not available | not available | Exp-cond_1 |
| 15N-filtered TOCSY (40 ms) | not available | not available | Exp-cond_1 |
| 15N-filtered TOCSY (60 ms) | not available | not available | Exp-cond_1 |
| 15N-13C filtered NOESY (80ms) | not available | not available | Exp-cond_1 |
Software:
NMRPipe v2 -
NMRView v5 -
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| EMBL | CAA38754 CAA38754 |
| PRF | 1616224A 1616224A |
| REF | NP_001001139 XP_005227654 XP_010841839 NP_001001139 |
| SP | Q03041 |
| TPG | DAA13266 |
| SWISS-PROT | Q03041 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts