BMRB Entry 5803
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5803
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Title: Three-disulfide variant of hen lysozyme: C64A/C80A PubMed: 15157100
Deposition date: 2003-05-19 Original release date: 2004-09-14
Authors: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi
Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins." Biochemistry 43, 6663-6669 (2004).
Assembly members:
three-disulfide variant of hen lysozyme, polymer, 130 residues, 14374 Da.
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
three-disulfide variant of hen lysozyme: MKVFGRCELAAAMKRHGLDN
YRGYSLGNWVCAAKFESNFN
TQATNRNTDGSTDYGILQIN
SRWWANDGRTPGSRNLCNIP
ASALLSSDITASVNCAKKIV
SDGNGMNAWVAWRNRCKGTD
VQAWIRGCRL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 680 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hen lysozyme Cys mutants, C64A/C80A | 1 |
Entities:
Entity 1, hen lysozyme Cys mutants, C64A/C80A 130 residues - 14374 Da.
| 1 | MET | LYS | VAL | PHE | GLY | ARG | CYS | GLU | LEU | ALA | |
| 2 | ALA | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | |
| 3 | TYR | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | |
| 4 | CYS | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | |
| 5 | THR | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | |
| 6 | SER | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | |
| 7 | SER | ARG | TRP | TRP | ALA | ASN | ASP | GLY | ARG | THR | |
| 8 | PRO | GLY | SER | ARG | ASN | LEU | CYS | ASN | ILE | PRO | |
| 9 | ALA | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | |
| 10 | ALA | SER | VAL | ASN | CYS | ALA | LYS | LYS | ILE | VAL | |
| 11 | SER | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | |
| 12 | ALA | TRP | ARG | ASN | ARG | CYS | LYS | GLY | THR | ASP | |
| 13 | VAL | GLN | ALA | TRP | ILE | ARG | GLY | CYS | ARG | LEU |
Samples:
sample_1: three-disulfide variant of hen lysozyme, [U-15N], 0.3 – 1.0 mM
EX-cond_1: pH: 3.8 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| HOHAHA | not available | not available | not available |
| 15N 3D-NOESY-HSQC | not available | not available | not available |
| 15N 3D-TOCSY-HSQC | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| 15N 3D-HSQC-NOESY-HSQC | not available | not available | not available |
Software:
XWINNMR v2.6 -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| SWISS-PROT | P00698 |
| GenBank | AAL69327 AAA48943 |
| PDB | |
| 1 | . . . . . . . . . . . . . . . . |
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| Hew | Lysozyme: |