BMRB Entry 6139
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6139
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Title: Solution Structure of Ole e 6, an allergen from olive tree pollen PubMed: 15247256
Deposition date: 2004-03-11 Original release date: 2004-08-05
Authors: Trevino, Miguel; Garcia-Mayoral, M Flor; Barral, Patricia; Villalba, Mayte; Rico, Manuel; Santoro, Jorge; Rodriguez, Rosalia; Bruix, Marta
Citation: Trevino, Miguel; Garcia-Mayoral, M. Flor; Barral, Patricia; Villalba, Mayte; Santoro, Jorge; Rico, Manuel; Rodriguez, Rosalia; Bruix, Marta. "NMR Solution Structure of Ole e 6, a major allergen from olive tree pollen" J. Biol. Chem. ., .-. (2004).
Assembly members:
Olea europea pollen allergen ole e 6, polymer, 50 residues, 5810 Da.
Natural source: Common Name: olive tree Taxonomy ID: 4146 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Olea europea
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
Olea europea pollen allergen ole e 6: DEAQFKECYDTCHKECSDKG
NGFTFCEMKCDTDCSVKDVK
EKLENYKPKN
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 52 |
| 1H chemical shifts | 614 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ole e 6 | 1 |
Entities:
Entity 1, ole e 6 50 residues - 5810 Da.
| 1 | ASP | GLU | ALA | GLN | PHE | LYS | GLU | CYS | TYR | ASP | |
| 2 | THR | CYS | HIS | LYS | GLU | CYS | SER | ASP | LYS | GLY | |
| 3 | ASN | GLY | PHE | THR | PHE | CYS | GLU | MET | LYS | CYS | |
| 4 | ASP | THR | ASP | CYS | SER | VAL | LYS | ASP | VAL | LYS | |
| 5 | GLU | LYS | LEU | GLU | ASN | TYR | LYS | PRO | LYS | ASN |
Samples:
sample_1: Olea europea pollen allergen ole e 60.6 – 1.0 mM; H2O 95%; D2O 5%
sample_2: Olea europea pollen allergen ole e 60.6 – 1.0 mM; D2O 100%
sample_3: Olea europea pollen allergen ole e 6, [U-15N], 0.6 – 1.0 mM; H2O 95%; D2O 5%
cond_pH6_H2O: pH: 6.0; temperature: 308 K
cond_pH6_D2O: pH: 6.0; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-COSY | not available | not available | not available |
| 1H-TOCSY | not available | not available | not available |
| 1H-NOESY | not available | not available | not available |
| 1H-15N-HSQC | not available | not available | not available |
| 1H-15N-NOESY-HSQC | not available | not available | not available |
Software:
XWINNMR - raw spectral data proccesing
ANSIG v3.3 - crosspeak area calculations, peak assigments, peak picking
CALIBA - upper limits calculations
DYANA v1.5 - structures calculations
GLOMSA - stereospecific assigments
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts