BMRB Entry 6175
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6175
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Title: 1H, 13C, and 15N assignments for the first TSR domain of F-spondin PubMed: 16736493
Deposition date: 2004-04-08 Original release date: 2007-11-05
Authors: Tossavainen, Helena
Citation: Paakkonen, Kimmo; Tossavainen, Helena; Permi, Perttu; Rakkolainen, Harri; Rauvala, Heikki; Raulo, Erkki; Kilpelainen, Ilkka; Guntert, Peter. "Solution structures of the first and fourth TSR domains of F-spondin" Proteins 64, 665-672 (2006).
Assembly members:
thrombospondin type-1 repeat, polymer, 61 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
thrombospondin type-1 repeat: GSETCIYSNWSPWSACSSST
CEKGKRMRQRMLKAQLDLSV
PCPDTQDFQPCMGPGCSDED
G
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 243 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 380 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | F-spondin TSR-1 | 1 |
Entities:
Entity 1, F-spondin TSR-1 61 residues - Formula weight is not available
| 1 | GLY | SER | GLU | THR | CYS | ILE | TYR | SER | ASN | TRP | ||||
| 2 | SER | PRO | TRP | SER | ALA | CYS | SER | SER | SER | THR | ||||
| 3 | CYS | GLU | LYS | GLY | LYS | ARG | MET | ARG | GLN | ARG | ||||
| 4 | MET | LEU | LYS | ALA | GLN | LEU | ASP | LEU | SER | VAL | ||||
| 5 | PRO | CYS | PRO | ASP | THR | GLN | ASP | PHE | GLN | PRO | ||||
| 6 | CYS | MET | GLY | PRO | GLY | CYS | SER | ASP | GLU | ASP | ||||
| 7 | GLY |
Samples:
sample_1: thrombospondin type-1 repeat, [U-13C; U-15N], 1.0 mM
Ex-cond_1: pH: 6.5; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | Ex-cond_1 |
| iHNCA | sample_1 | not available | Ex-cond_1 |
| HN(CO)CA | sample_1 | not available | Ex-cond_1 |
| HNCO | sample_1 | not available | Ex-cond_1 |
| HN(CA)CO | sample_1 | not available | Ex-cond_1 |
| HNCACB | sample_1 | not available | Ex-cond_1 |
| HN(CO)CACB | sample_1 | not available | Ex-cond_1 |
| CC(CO)NH | sample_1 | not available | Ex-cond_1 |
| H(CCO)NH | sample_1 | not available | Ex-cond_1 |
| HCCH-COSY | sample_1 | not available | Ex-cond_1 |
Software:
VNMR v6.1 C - processing
SPARKY v3.1 - analysis, peak picking
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts