BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6211

Title: Chemical Shift Assignments for Carnobacteriocin B2 immunity protein   PubMed: 15362858

Authors: Sprules, Tara; Kawulka, Karen; Vederas, John

Citation: Sprules, Tara; Kawulka, Karen; Vederas, John. "NMR Solution Structure of ImB2, a Protein Conferring Immunity to Antimicrobial Activity of the Type IIa Bacteriocin, Carnobacteriocin B2 "  Biochemistry 43, 11740-11749 (2004).

Assembly members:
Carnobacteriocin B2 immunity protein, polymer, 111 residues, 12666 Da.

Natural source:   Common Name: Carnobacterium maltaromaticum   Taxonomy ID: 2751   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Carnobacterium piscicola

Experimental source:   Production method: recombinant technology   Host organism: Esherichia coli

Entity Sequences (FASTA):
Carnobacteriocin B2 immunity protein: MDIKSQTLYLNLSEAYKDPE VKANEFLSKLVVQCAGKLTA SNSENSYIEVISLLSRGISS YYLSHKRIIPSSMLTIYTQI QKDIKNGNIDTEKLRKYEIA KGLMSVPYIYF

Data sets:
Data typeCount
1H chemical shifts748
13C chemical shifts470
15N chemical shifts112
coupling constants71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1imB21

Entities:

Entity 1, imB2 111 residues - 12666 Da.

1   METASPILELYSSERGLNTHRLEUTYRLEU
2   ASNLEUSERGLUALATYRLYSASPPROGLU
3   VALLYSALAASNGLUPHELEUSERLYSLEU
4   VALVALGLNCYSALAGLYLYSLEUTHRALA
5   SERASNSERGLUASNSERTYRILEGLUVAL
6   ILESERLEULEUSERARGGLYILESERSER
7   TYRTYRLEUSERHISLYSARGILEILEPRO
8   SERSERMETLEUTHRILETYRTHRGLNILE
9   GLNLYSASPILELYSASNGLYASNILEASP
10   THRGLULYSLEUARGLYSTYRGLUILEALA
11   LYSGLYLEUMETSERVALPROTYRILETYR
12   PHE

Samples:

sample_1: Carnobacteriocin B2 immunity protein, [U-15N], 0.8 mM; DSS 0.025 mM; sodium phosphate 20 mM; sodium chloride 25 mM

sample_2: Carnobacteriocin B2 immunity protein, [U-13C; U-15N], 0.8 mM; DSS 0.025 mM; sodium phosphate 20 mM; sodium chloride 25 mM

condition_1: pH: 6.6; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQC NOESYnot availablenot availablecondition_1
15N-HSQC TOCSYnot availablenot availablecondition_1
13C-HSQC NOESYnot availablenot availablecondition_1
13C-HSQC TOCSYnot availablenot availablecondition_1
HNHAnot availablenot availablecondition_1
13C-HSQCnot availablenot availablecondition_1
HNCACBnot availablenot availablecondition_1
HNCAnot availablenot availablecondition_1
HNCOnot availablenot availablecondition_1
CBCACONHnot availablenot availablecondition_1
15N-HSQCnot availablenot availablecondition_1

Software:

NMRPipe v2.2 - data processing

NMRView v5.0 - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAA72432 AAB18990 AAB81311
SP P38582