BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6217

Title: Antibiotic Activity and Structural Analysis of a Scorpion-derived Antimicrobial peptide IsCT and Its Analogs   PubMed: 15369808

Authors: Lee, K.; Shin, S.; Kim, K.; Lim, S.; Hahm, K.; Kim, Y.

Citation: Lee, K.; Shin, S.; Kim, K.; Lim, S.; Hahm, K.; Kim, Y.. "Antibiotic Activity and Structural Analysis of the Scorpion-derived Antimicrobial peptide IsCT and Its Analogs"  Biochem. Biophys. Res. Commun. 323, 712-719 (2004).

Assembly members:
IsCT, polymer, 13 residues, Formula weight is not available
NFA, non-polymer, 164.204 Da.

Natural source:   Common Name: Scorpion   Taxonomy ID: 167108   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Opisthacanthus madagascariensis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IsCT: ILGKIWKPIKKLX

Data sets:
Data typeCount
coupling constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cytotoxic linear peptide IsCT1
2NFA2

Entities:

Entity 1, Cytotoxic linear peptide IsCT 13 residues - Formula weight is not available

1   ILELEUGLYLYSILETRPLYSPROILELYS
2   LYSLEUNFA

Entity 2, NFA - C9 H12 N2 O - 164.204 Da.

1   NFA

Samples:

sample_1: IsCT 1.0 mM; SDS-d25 200 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1

Software:

FELIX v2000.1 - processing

X-PLOR v3.851 - structure solution

DGII v2000.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 400 MHz

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