BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6221

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments of the Resuscitation Promoting Factor (Rpf) domain of M. tuberculosis   PubMed: 15614636

Authors: Cohen-Gonsaud, Martin; Barthe, Philippe; Keep, Nicholas; Roumestand, Christian

Citation: Cohen-Gonsaud, Martin; Barthe, Philippe; Pommier, Francoise; Harris, Richard; Driscoll, Paul; Keep, Nicholas; Roumestand, Christian. "1H, 15N and 13C Chemical Shifts Assignments of the Resuscitation Promoting Factor domain of Rv1009 from Mycobacterium tuberculosis"  J. Biomol. NMR 30, 373-374 (2004).

Assembly members:
Rpf domain, polymer, 108 residues, Formula weight is not available

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 83332   Superkingdom: Actinobacteria   Kingdom: Actinomycetales   Genus/species: Mycobacterium tuberculosis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Rpf domain: NVVVTPAHEAVVRVGTKPGT EVPPVIDGSIWDAIAGCEAG GNWAINTGNGYYGGVQFDQG TWEANGGLRYAPRADLATRE EQIAVAEVTRLRQGWGAWPV CAARAGAR

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts124
1H chemical shifts677

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RpfBc domain from M. tuberculosis1

Entities:

Entity 1, RpfBc domain from M. tuberculosis 108 residues - Formula weight is not available

1   ASNVALVALVALTHRPROALAHISGLUALA
2   VALVALARGVALGLYTHRLYSPROGLYTHR
3   GLUVALPROPROVALILEASPGLYSERILE
4   TRPASPALAILEALAGLYCYSGLUALAGLY
5   GLYASNTRPALAILEASNTHRGLYASNGLY
6   TYRTYRGLYGLYVALGLNPHEASPGLNGLY
7   THRTRPGLUALAASNGLYGLYLEUARGTYR
8   ALAPROARGALAASPLEUALATHRARGGLU
9   GLUGLNILEALAVALALAGLUVALTHRARG
10   LEUARGGLNGLYTRPGLYALATRPPROVAL
11   CYSALAALAARGALAGLYALAARG

Samples:

sample_1: Rpf domain, [U-95% 15N], 0.5 mM; Na-Acetate 25 mM; beta-mercaptoethanol 5 mM

sample_2: Rpf domain, [U-95% 13C; U-95% 15N], 0.5 mM; Na-Acetate 25 mM; beta-mercaptoethanol 5 mM

Ex-cond_1: ionic strength: 25 mM; pH: 4.6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableEx-cond_1
HN(CO)CAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HN(CA)COnot availablenot availableEx-cond_1
1H-15N NOESYnot availablenot availableEx-cond_1
1H-15N TOCSYnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAH25329
EMBL CAB08136 CAD93897 CAL71053
GB AAK45288 ABQ72752 ABR05371 ACT26067 EAY59373
REF NP_215525 NP_335474 NP_854693 YP_001282314 YP_001286973