BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6222

Title: Solution Structure of Kurtoxin

Authors: Lee, C.; Min, H.; Cho, E.; Kohno, T.; Eu, Y.; Kim, J.

Citation: Lee, C.; Min, H.; Cho, E.; Kohno, T.; Eu, Y.; Kim, J.. "Solution Structure of Kurtoxin"  . ., .-..

Assembly members:
scorpion toxin, polymer, 63 residues, Formula weight is not available

Natural source:   Common Name: South African fattail scorpion   Taxonomy ID: 170972   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Parabuthus Parabuthus transvaalicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
scorpion toxin: KIDGYPVDYWNCKRICWYNN KYCNDLCKGLKADSGYCWGW TLSCYCQGLPDNARIKRSGR CRA

Data sets:
Data typeCount
1H chemical shifts432

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kurtoxin1

Entities:

Entity 1, Kurtoxin 63 residues - Formula weight is not available

1   LYSILEASPGLYTYRPROVALASPTYRTRP
2   ASNCYSLYSARGILECYSTRPTYRASNASN
3   LYSTYRCYSASNASPLEUCYSLYSGLYLEU
4   LYSALAASPSERGLYTYRCYSTRPGLYTRP
5   THRLEUSERCYSTYRCYSGLNGLYLEUPRO
6   ASPASNALAARGILELYSARGSERGLYARG
7   CYSARGALA

Samples:

sample_1: scorpion toxin, [U-15N; U-13C], 1 mM; CD3CN 25%; D2O 10%; H2O 65%

sample_condition_1: ionic strength: 0 M; pH: 4.0; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

AZARA v2.5 - processing

ANSIG v3.3 - data analysis

X-PLOR v3.851 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
SWISS-PROT P0C5F1 P58910