BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6247

Title: Solution structure of C-terminal domain of NifU-like protein from Oryza sativa   PubMed: 15978837

Authors: Kumeta, H.; Ogura, K.; Asayama, M.; Katoh, S.; Katoh, E.; Inagaki, F.

Citation: Katoh, S.; Murata, K.; Kubota, Y.; Kumeta, H.; Ogura, K.; Inagaki, F.; Asayama, M.; Katoh, E.. "Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli."  Protein Expr. Purif. 43, 149-156 (2005).

Assembly members:
NifU1, polymer, 74 residues, Formula weight is not available

Natural source:   Common Name: rice   Taxonomy ID: 4530   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oryza sativa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NifU1: MLELNEENVEKVLNEIRPYL AGTGGGGLQFLMIKGPIVKV RLTGPAAVVRTVRIAVSKKL REKIPSIQIVQLLS

Data sets:
Data typeCount
13C chemical shifts344
15N chemical shifts80
1H chemical shifts547

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NifU11

Entities:

Entity 1, NifU1 74 residues - Formula weight is not available

1   METLEUGLULEUASNGLUGLUASNVALGLU
2   LYSVALLEUASNGLUILEARGPROTYRLEU
3   ALAGLYTHRGLYGLYGLYGLYLEUGLNPHE
4   LEUMETILELYSGLYPROILEVALLYSVAL
5   ARGLEUTHRGLYPROALAALAVALVALARG
6   THRVALARGILEALAVALSERLYSLYSLEU
7   ARGGLULYSILEPROSERILEGLNILEVAL
8   GLNLEULEUSER

Samples:

sample_1: NifU1, [U-15N], 0.8 mM; NaPi 20 mM; NaCl 100 mM; beta-ME 2.5 mM; H2O 90%; D2O 10%

sample_2: NifU1, [U-13C; U-15N], 0.45 mM; NaPi 20 mM; NaCl 100 mM; beta-ME 2.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1c - collection

NMRPipe v23 - processing

SPARKY v3.11 - data analysis

CYANA v1.06 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

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