BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6254

Title: 1H, 13C, and 15N Chemical Shift Assignments for I14A mutant of histidine-containing phosphocarrier protein from Staphylococcus carnosus   PubMed: 15606769

Authors: Moglich, Andreas; Koch, Brigitte; Hengstenberg, Wolfgang; Brunner, Eike; Kalbitzer, Hans Robert

Citation: Moglich, Andreas; Koch, Brigitte; Gronwald, W.; Hengstenberg, Wolfgang; Brunner, Eike; Kalbitzer, Hans Robert. "Solution structure of the active-centre mutant I14A of the histidine-containing phosphocarrier protein from Staphylococcus carnosus"  Eur. J. Biochem. 271, 4815-4824 (2004).

Assembly members:
HPrI14A from S. carnosus, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus carnosus   Taxonomy ID: 1281   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus carnosus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HPrI14A from S. carnosus: MEQQSYTIIDETGAHARPAT MLVQTASKFDSDIQLEYNGK KVNLKSIMGVMSLGVGKDAE ITIYADGSDEADAIQAITDV LSKEGLTE

Data sets:
Data typeCount
1H chemical shifts598
13C chemical shifts269
15N chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HPr1

Entities:

Entity 1, HPr 88 residues - Formula weight is not available

1   METGLUGLNGLNSERTYRTHRILEILEASP
2   GLUTHRGLYALAHISALAARGPROALATHR
3   METLEUVALGLNTHRALASERLYSPHEASP
4   SERASPILEGLNLEUGLUTYRASNGLYLYS
5   LYSVALASNLEULYSSERILEMETGLYVAL
6   METSERLEUGLYVALGLYLYSASPALAGLU
7   ILETHRILETYRALAASPGLYSERASPGLU
8   ALAASPALAILEGLNALAILETHRASPVAL
9   LEUSERLYSGLUGLYLEUTHRGLU

Samples:

sample_1: HPrI14A from S. carnosus, [U-15N], 1.7 mM; potassium phosphate 20 mM; potassium chloride 100 mM

sample_2: HPrI14A from S. carnosus, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; potassium chloride 100 mM

Ex-cond_1: pH: 7.14; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D-TOCSYnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HSQCnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 800 MHz

Related Database Links:

PDB
EMBL CAA43175 CAL27615
GB AAA26663 EKS26159 ERS92838
REF WP_002480512 WP_015899958 YP_002633800
SP P23534