BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6264

Title: Solution structure of cryptdin-4, the most potent alpha-defensin from mouse Paneth cells   PubMed: 15595831

Authors: Jing, W.; Hunter, H.; Tanabe, H.; Ouellette, A.; Vogel, H.

Citation: Jing, W.; Hunter, H.; Tanabe, H.; Ouellette, A.; Vogel, H.. "Solution structure of cryptdin-4, a mouse paneth cell alpha-defensin"  Biochemistry 43, 15759-15766 (2004).

Assembly members:
Cryptdin-4, polymer, 32 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cryptdin-4: GLLCYCRKGHCKRGERVRGT CGIRFLYCCPRR

Data sets:
Data typeCount
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cryptdin-41

Entities:

Entity 1, Cryptdin-4 32 residues - Formula weight is not available

1   GLYLEULEUCYSTYRCYSARGLYSGLYHIS
2   CYSLYSARGGLYGLUARGVALARGGLYTHR
3   CYSGLYILEARGPHELEUTYRCYSCYSPRO
4   ARGARG

Samples:

sample_1: Cryptdin-4 3.2 mg

sample_cond_1: pH: 4.2; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

NMRView v4.1.3 - data analysis

CNS - refinement

ARIA v1.2 - structure solution

NMR spectrometers:

  • Bruker Advance 700 MHz

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