BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6265

Title: Structure and Biochemical Function of a Prototypical Arabidopsis U-box Domain   PubMed: 15231834

Authors: Andersen, P.; Kragelund, B.; Olsen, A.; Larsen, F.; Chua, N.-H.; Poulsen, F.; Skriver, K.

Citation: Andersen, P.; Kragelund, B.; Olsen, A.; Larsen, F.; Chua, N.-H.; Poulsen, F.; Skriver, K.. "Structure and Biochemical Function of a Prototypical Arabidopsis U-box Domain"  J. Biol. Chem. 279, 40053-40061 (2004).

Assembly members:
PUB14, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: thale cress   Taxonomy ID: 8754   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PUB14: GSPEFPEYFRCPISLELMKD PVIVSTGQTYERSSIQKWLD AGHKTCPKSQETLLHAGLTP NYVLKSLIALWCESNGIE

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts63
1H chemical shifts498

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PUB141

Entities:

Entity 1, PUB14 78 residues - Formula weight is not available

1   GLYSERPROGLUPHEPROGLUTYRPHEARG
2   CYSPROILESERLEUGLULEUMETLYSASP
3   PROVALILEVALSERTHRGLYGLNTHRTYR
4   GLUARGSERSERILEGLNLYSTRPLEUASP
5   ALAGLYHISLYSTHRCYSPROLYSSERGLN
6   GLUTHRLEULEUHISALAGLYLEUTHRPRO
7   ASNTYRVALLEULYSSERLEUILEALALEU
8   TRPCYSGLUSERASNGLYILEGLU

Samples:

sample_1: PUB14, [U-15N; U-13C], 0.9 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 10%; H20 90%

sample_2: PUB14, [U-15N; U-13C], 0.6 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 10%; H20 90%

sample_3: PUB14, [U-15N], 0.6 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 10%; H20 90%

sample_4: PUB14, [U-15N; U-13C], 0.4 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 99.96%; H20 0.04%

sample_cond_1: ionic strength: 0.17 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated TOCSYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

NMRPipe - processing

PRONTO v20020517 - data analysis

CYANA - structure calculation

X-PLOR vV3.840 - structure calculation

CNS v1.1 - refinement

NMR spectrometers:

  • Varian UNITY INOVA 800 MHz

Related Database Links:

PDB
DBJ BAH57001
GB AAL38755 AAM20180 AEE79300 EFH52534
REF NP_191045 XP_002876275 XP_010427209 XP_010504312 XP_010516027
SP Q8VZ40