BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6269

Title: 1H, 13C, and 15N Chemical Shift Assignments for chPrP(128-242)   PubMed: 15459548

Authors: Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt

Citation: Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt. "Letters to the Editor: NMR assignment of the chicken prion protein fragments chPrP(128-242) and chPrP(25-242)"  J. Biomol. NMR 30, 97-97 (2004).

Assembly members:
chicken prion protein, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
chicken prion protein: GSVVGGLGGYAMGRVMSGMN YHFDRPDEYRWWSENSARYP NRVYYRDYSSPVPQDVFVAD CFNITVTEYSIGPAAKKNTS EAVAAANQTEVEMENKVVTK VIREMCVQQYREYRLAS

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts135
1H chemical shifts804

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1chPrP (128-242)1

Entities:

Entity 1, chPrP (128-242) 117 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   ALAMETGLYARGVALMETSERGLYMETASN
3   TYRHISPHEASPARGPROASPGLUTYRARG
4   TRPTRPSERGLUASNSERALAARGTYRPRO
5   ASNARGVALTYRTYRARGASPTYRSERSER
6   PROVALPROGLNASPVALPHEVALALAASP
7   CYSPHEASNILETHRVALTHRGLUTYRSER
8   ILEGLYPROALAALALYSLYSASNTHRSER
9   GLUALAVALALAALAALAASNGLNTHRGLU
10   VALGLUMETGLUASNLYSVALVALTHRLYS
11   VALILEARGGLUMETCYSVALGLNGLNTYR
12   ARGGLUTYRARGLEUALASER

Samples:

sample_1: chicken prion protein, [U-13C; U-15N], 1.0 mM; sodium acetate 1.0 mM

Ex-cond_1: ionic strength: 10 mM; pH: 4.3; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-13N NOESY aliphatic, aromaticnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
GenBank AAA49041 AAC28970 AAR21237
REF NP_990796
SWISS-PROT P27177