BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6284

Title: 1H, 13C, and 15N Chemical Shift Assignments for DnaG-C   PubMed: 15649896

Authors: Oakley, Aaron; Loscha, Karin; Schaeffer, Patrick; Liepinsh, Edvards; Pintacuda, Guido; Wilce, Matthew; Otting, Gottfried; Dixon, Nicholas

Citation: Oakley, Aaron; Loscha, Karin; Schaeffer, Patrick; Liepinsh, Edvards; Pintacuda, Guido; Wilce, Matthew; Otting, Gottfried; Dixon, Nicholas. "Crystal and solution structures of the helicase-binding domain of Escherichia coli primase"  J. Biol. Chem. 280, 11495-11504 (2005).

Assembly members:
DnaG-C, polymer, 148 residues, 16690 Da.

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
DnaG-C: AAESGVSRPVPQLKRTTMRI LIGLLVQNPELATLVPPLEN LDENKLPGLGLFRELVNTCL SQPGLTTGQLLEHYRGTNNA ATLEKLSMWDDIADKNIAEQ TFTDSLNHMFDSLLELRQEE LIARERTHGLSNEERLELWT LNQELAKK

Data sets:
Data typeCount
13C chemical shifts757
15N chemical shifts300
1H chemical shifts1278

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaG-C1

Entities:

Entity 1, DnaG-C 148 residues - 16690 Da.

1   ALAALAGLUSERGLYVALSERARGPROVAL
2   PROGLNLEULYSARGTHRTHRMETARGILE
3   LEUILEGLYLEULEUVALGLNASNPROGLU
4   LEUALATHRLEUVALPROPROLEUGLUASN
5   LEUASPGLUASNLYSLEUPROGLYLEUGLY
6   LEUPHEARGGLULEUVALASNTHRCYSLEU
7   SERGLNPROGLYLEUTHRTHRGLYGLNLEU
8   LEUGLUHISTYRARGGLYTHRASNASNALA
9   ALATHRLEUGLULYSLEUSERMETTRPASP
10   ASPILEALAASPLYSASNILEALAGLUGLN
11   THRPHETHRASPSERLEUASNHISMETPHE
12   ASPSERLEULEUGLULEUARGGLNGLUGLU
13   LEUILEALAARGGLUARGTHRHISGLYLEU
14   SERASNGLUGLUARGLEUGLULEUTRPTHR
15   LEUASNGLNGLULEUALALYSLYS

Samples:

sample_1: DnaG-C, [U-90% 13C; U-15N], 0.6 mM; phosphate buffer 10 mM; DTT 1 mM; NaCl 100 mM; NaN3 0.1%

sample_2: DnaG-C, [U-90% 13C; U-15N], 0.4 mM; phosphate buffer 10 mM; DTT 1 mM; NaCl 100 mM; NaN3 0.1%

sample_conditions: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions

Software:

xwinnmr v2.8 - peak assignments

PROSA v2.8 - spectrum processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAB37372 BAE77117 BAG78871 BAI27347 BAI32464
EMBL CAA23531 CAP77540 CAQ33403 CAQ90500 CAR00028
GB AAA24600 AAA89146 AAC76102 AAG58200 AAN44583
PIR D85967
PRF 0902269A
REF NP_289641 NP_311976 NP_417538 NP_708876 NP_755688
SP P0ABS5 P0ABS6 P0ABS7 Q8FDG5