BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6286

Title: 1H and 15N assignment of SMRT DAD   PubMed: 15837933

Authors: Codina, Anna; Schwabe, John

Citation: Codina, Anna; Love, James; Li, Y.; Lazar, M.; Neuhaus, David; Schwabe, John. "From The Cover: Structural insights into the interaction and activation of histone deacetylase 3 by nuclear receptor corepressors."  Proc. Natl. Acad. Sci. U. S. A. 102, 6009-6014 (2005).

Assembly members:
SMRT deacetylase activation domain, polymer, 68 residues, 8291.9 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SMRT deacetylase activation domain: NGLMADPMKVYKDRQVMNMW SEQEKETFREKFMQHPKNFG LIASFLERKTVAECVLYYYL TKKNENYK

Data sets:
Data typeCount
15N chemical shifts73
1H chemical shifts403

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SMRT DAD1

Entities:

Entity 1, SMRT DAD 68 residues - 8291.9 Da.

1   ASNGLYLEUMETALAASPPROMETLYSVAL
2   TYRLYSASPARGGLNVALMETASNMETTRP
3   SERGLUGLNGLULYSGLUTHRPHEARGGLU
4   LYSPHEMETGLNHISPROLYSASNPHEGLY
5   LEUILEALASERPHELEUGLUARGLYSTHR
6   VALALAGLUCYSVALLEUTYRTYRTYRLEU
7   THRLYSLYSASNGLUASNTYRLYS

Samples:

sample_1: SMRT deacetylase activation domain1 – 2 mM; NaCl 50 mM; sodium phosphate 50 mM

sample_2: SMRT deacetylase activation domain, [U-15N], 1 – 2 mM; NaCl 50 mM; sodium phosphate 50 mM

cond_1: ionic strength: 0.350 M; pH: 6.8; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
(1H, 1H) DQF-COSYnot availablenot availablecond_1
(1H, 1H) DQ-correlationnot availablenot availablecond_1
(1H, 1H) NOESYnot availablenot availablecond_1
(1H, 1H) TOCSYnot availablenot availablecond_1
2D 15N-HSQCnot availablenot availablecond_1
3D 15N-NOESY-HSQCnot availablenot availablecond_1
3D 15N-TOCSY-HSQCnot availablenot availablecond_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAB91446
REF XP_001102940 XP_004310483 XP_006751297 XP_006938465 XP_009180278
TPG DAA20733