BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6289

Title: NMR Structure of the Free Zinc Binding C-terminal Domain of SecA   PubMed: 15488768

Authors: Matousek, William; Alexandrescu, Andrei

Citation: Matousek, William; Alexandrescu, Andrei. "NMR structure of the C-terminal domain of SecA in the free state."  Biochim. Biophys. Acta 1702, 163-171 (2004).

Assembly members:
SecA_Ct, polymer, 22 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
SecA_Ct: GRNDPCPCGSGKKYKQCHGR LQ

Data sets:
Data typeCount
13C chemical shifts55
15N chemical shifts18
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SecA_Ct1
2Zinc ion2

Entities:

Entity 1, SecA_Ct 22 residues - Formula weight is not available

1   GLYARGASNASPPROCYSPROCYSGLYSER
2   GLYLYSLYSTYRLYSGLNCYSHISGLYARG
3   LEUGLN

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: SecA_Ct 2.5 mM; zinc chloride 2.7 mM; potassium phosphate 10 mM; H2O 90%; D2O 10%

sample_condition_1: ionic strength: 0.01 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

sample_condition_2: ionic strength: 0.01 M; pH: 6.03; pressure: 1 atm; temperature: 298 K

sample_condition_3: ionic strength: 0.01 M; pH: 6.80; pressure: 1 atm; temperature: 298 K

sample_condition_4: ionic strength: 0.01 M; pH: 6.43; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H NOESYsample_1not availablenot available
2D 1H TOCSYsample_1not availablenot available
natural abundance 1H15N HSQCsample_1not availablenot available
1D hydrogen exchangesample_1not availablenot available
natural abundance 1H13C HSQCsample_1not availablenot available
1H 2D ECOSYsample_1not availablenot available
1H 2D phase sensitive COSYsample_1not availablenot available

Software:

VNMR -

FELIX v2000 -

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

SP A4G8S7 A4TQ74 A1JJK2 A2SJF9 A1A7E3
REF NP_454748 NP_308129 NP_414640 AP_000761 NP_285794
GB AAM87165 AAG54402 AAL19100 AAA24619 AAC73209
PIR AE0519
EMBL CAH19937 CAL10790 CAD01293 CAG76705 CAA38875
DBJ BAI23462 BAG75624 BAH61745 BAB33525 BAB96666