BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6299

Title: Structure of a Chordin-like Cysteine-rich Repeat (VWC module) from Collagen IIA   PubMed: 15466413

Authors: O'Leary, J.; Hamilton, J.; Deane, C.; Valeyev, N.; Sandell, L.; Downing, A.

Citation: O'Leary, J.; Hamilton, J.; Deane, C.; Valeyev, N.; Sandell, L.; Downing, A.. "Solution Structure and Dynamics of a Prototypical Chordin-like Cysteine-rich Repeat (von Willebrand Factor Type C Module) from Collagen IIA"  J. Biol. Chem. 279, 53857-53866 (2004).

Assembly members:
alpha 1 type II collagen isoform 1, polymer, 73 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
alpha 1 type II collagen isoform 1: YVEFQEAGSCVQDGQRYNDK DVWKPEPCRICVCDTGTVLC DDIICEDVKDCLSPEIPFGE CCPICPADLAAAA

Data sets:
Data typeCount
13C chemical shifts177
15N chemical shifts65
1H chemical shifts414

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha 1 type II collagen isoform 11

Entities:

Entity 1, alpha 1 type II collagen isoform 1 73 residues - Formula weight is not available

1   TYRVALGLUPHEGLNGLUALAGLYSERCYS
2   VALGLNASPGLYGLNARGTYRASNASPLYS
3   ASPVALTRPLYSPROGLUPROCYSARGILE
4   CYSVALCYSASPTHRGLYTHRVALLEUCYS
5   ASPASPILEILECYSGLUASPVALLYSASP
6   CYSLEUSERPROGLUILEPROPHEGLYGLU
7   CYSCYSPROILECYSPROALAASPLEUALA
8   ALAALAALA

Samples:

sample_1: alpha 1 type II collagen isoform 1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_2: alpha 1 type II collagen isoform 1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; D2O 99.9%

sample_3: alpha 1 type II collagen isoform 1, [U-13C; U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
HMQC-Jnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

CYANA v1.0.6 - refinement, structure solution

NMR spectrometers:

  • GE Omega 750 MHz

Related Database Links:

PDB