BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6300

Title: Solution structure of rat Kalirin N-terminal SH3 domain   PubMed: 16644733

Authors: Schiller, M.; Chakrabarti, K.; King, G.; Schiller, N.; Eipper, B.; Maciejewski, M.

Citation: Schiller, M.; Chakrabarti, K.; King, G.; Schiller, N.; Eipper, B.; Maciejewski, M.. "Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions"  J. Biol. Chem. 281, 18774-18786 (2006).

Assembly members:
Kalirin, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: 10116   Taxonomy ID: Norway rat   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Kalirin: GPLGSPGIPGSTLSGGCELT VVLQDFSAAHSSELSIQVGQ TVELLERPSERPGWCLVRTT ERSPPQEGLVPSSTLCISHS RS

Data sets:
Data typeCount
13C chemical shifts314
15N chemical shifts78
1H chemical shifts502

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kalirin1

Entities:

Entity 1, Kalirin 82 residues - Formula weight is not available

1   GLYPROLEUGLYSERPROGLYILEPROGLY
2   SERTHRLEUSERGLYGLYCYSGLULEUTHR
3   VALVALLEUGLNASPPHESERALAALAHIS
4   SERSERGLULEUSERILEGLNVALGLYGLN
5   THRVALGLULEULEUGLUARGPROSERGLU
6   ARGPROGLYTRPCYSLEUVALARGTHRTHR
7   GLUARGSERPROPROGLNGLUGLYLEUVAL
8   PROSERSERTHRLEUCYSILESERHISSER
9   ARGSER

Samples:

sample_1: Kalirin, [U-15N], 0.4 mM; HEPES 50 mM; NaCl 150 mM; dithiotheitol 1 mM; H2O 92.5%; D2O 7.5%

sample_2: Kalirin, [U-13C; U-15N], 1 mM; HEPES 50 mM; NaCl 150 mM; dithiotheitol 1 mM; H2O 92.5%; D2O 7.5%

sample_cond_1: ionic strength: 200 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
3D HN(CO)CACBnot availablenot availablenot available
3D C(CO)NH-TOCSYnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 13C HSQC-NOESYnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
HC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available

Software:

DYANA v1.5 - structure solution

CYANA v1.0.0 - structure solution

X-PLOR v3.851 - refinement

NMRPipe v2.3 - processing

XEASY v1.4 - data analysis

TALOS - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

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