BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6301

Title: NMR solution structure of a designed heterodimeric leucine zipper   PubMed: 15449933

Authors: Marti, D.; Bosshard, H.

Citation: Marti, D.; Bosshard, H.. "Inverse electrostatic effect: electrostatic repulsion in the unfolded state stabilizes a leucine zipper"  Biochemistry 43, 12436-12447 (2004).

Assembly members:
LEUCINE ZIPPER CHAIN A, polymer, 32 residues, 3496 Da.
LEUCINE ZIPPER CHAIN B, polymer, 32 residues, 3515 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
LEUCINE ZIPPER CHAIN A: XEVAQLEKEVAQLEAENYQL EQEVAQLEHEGX
LEUCINE ZIPPER CHAIN B: XEVQALKKRVQALKARNYAL KQKVQALRHKGX

Data sets:
Data typeCount
15N chemical shifts73
1H chemical shifts392
coupling constants57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACIDIC CHAIN1
2BASIC CHAIN2

Entities:

Entity 1, ACIDIC CHAIN 32 residues - 3496 Da.

1   ACEGLUVALALAGLNLEUGLULYSGLUVAL
2   ALAGLNLEUGLUALAGLUASNTYRGLNLEU
3   GLUGLNGLUVALALAGLNLEUGLUHISGLU
4   GLYNH2

Entity 2, BASIC CHAIN 32 residues - 3515 Da.

1   ACEGLUVALGLNALALEULYSLYSARGVAL
2   GLNALALEULYSALAARGASNTYRALALEU
3   LYSGLNLYSVALGLNALALEUARGHISLYS
4   GLYNH2

Samples:

sample_1: LEUCINE ZIPPER CHAIN A 2.6 mM; LEUCINE ZIPPER CHAIN B 2.6 mM; NaCl 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 10 mM; pH: 5.7; pressure: 1 atm; temperature: 310.00 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
2D 1H-15N HSQCsample_1not availablesample_cond_1
2D 1H-13C HSQCsample_1not availablesample_cond_1

Software:

xwinnmr v3.0 - data collection

FELIX v2000 - data analysis

CNS v1.1 - refinement, structure solution

Procheck v3.4.4 - data analysis

WhatIF v19991018 - data analysis

NMR spectrometers:

  • BRUKER DRX 600 MHz

Related Database Links:

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