BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6326

Title: Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting   PubMed: 15698569

Authors: Sharpe, B.; Liew, C.; Wilce, J.; Crossley, M.; Matthews, J.; Mackay, J.

Citation: Sharpe, B.; Liew, C.; Kwan, A.; Wilce, J.; Crossley, M.; Matthews, J.; Mackay, J.. "Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting"  Structure 13, 257-266 (2005).

Assembly members:
CREB Binding Protein (E.C.2.3.1.48), polymer, 25 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: Chemically synthesized

Entity Sequences (FASTA):
CREB Binding Protein (E.C.2.3.1.48): AVYYCILPKCAAAANVAAHT THCFK

Data sets:
Data typeCount
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CREB Binding Protein1
2ZINC (II) ION2

Entities:

Entity 1, CREB Binding Protein 25 residues - Formula weight is not available

1   ALAVALTYRTYRCYSILELEUPROLYSCYS
2   ALAALAALAALAASNVALALAALAHISTHR
3   THRHISCYSPHELYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CREB Binding Protein (E.C.2.3.1.48) 0.3 mM; TCEP 0.5 mM; ZnSO4 1 mM; D2O 5%; H2O 95%

sample_cond_1: pH: 7.2; pressure: 1 atm; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1

Software:

xwinnmr v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - data analysis

CNS v1.0 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

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