BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6333

Title: 1H, 13C, and 15N chenical shift assignments for ABA-1A with oleic acid bound

Authors: Meenan, Nicola; Cooper, Alan; Kennedy, Malcolm; Smith, Brian

Citation: Meenan, Nicola; Cooper, Alan; Kennedy, Malcolm; Smith, Brian. "Resonance assignment of ABA-1A, from Ascaris suum nematode polyprotein allergen"  J. Biomol. NMR 32, 176-176 (2005).

Assembly members:
ABA-1A, polymer, 134 residues, Formula weight is not available
OLEIC ACID, non-polymer, 282.461 Da.

Natural source:   Common Name: Ascaris suum   Taxonomy ID: 6253   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Ascaris suum

Experimental source:   Production method: recombinant technology   Host organism: coli

Entity Sequences (FASTA):
ABA-1A: GSPEFHHFTLESSLDTHLKW LSQEQKDELLKMKKDGKAKK ELEAKILHYYDELEGDAKKE ATEHLKGGCREILKHVVGEE KAAELKNLKDSGASKEELKA KVEEALHAVTDEEKKQYIAD FGPACKKIYGVHTS

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts129
1H chemical shifts854

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ABA-1A1
2oleic acid2

Entities:

Entity 1, ABA-1A 134 residues - Formula weight is not available

1   GLYSERPROGLUPHEHISHISPHETHRLEU
2   GLUSERSERLEUASPTHRHISLEULYSTRP
3   LEUSERGLNGLUGLNLYSASPGLULEULEU
4   LYSMETLYSLYSASPGLYLYSALALYSLYS
5   GLULEUGLUALALYSILELEUHISTYRTYR
6   ASPGLULEUGLUGLYASPALALYSLYSGLU
7   ALATHRGLUHISLEULYSGLYGLYCYSARG
8   GLUILELEULYSHISVALVALGLYGLUGLU
9   LYSALAALAGLULEULYSASNLEULYSASP
10   SERGLYALASERLYSGLUGLULEULYSALA
11   LYSVALGLUGLUALALEUHISALAVALTHR
12   ASPGLUGLULYSLYSGLNTYRILEALAASP
13   PHEGLYPROALACYSLYSLYSILETYRGLY
14   VALHISTHRSER

Entity 2, oleic acid - C18 H34 O2 - 282.461 Da.

1   OLA

Samples:

sample: ABA-1A, [U-15N; U-13C], 2 mM; OLEIC ACID 2 mM; phosphate buffer 50 mM; sodium chloride 50 mM

sample_conditions: pH*: 7; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCsamplenot availablesample_conditions
13C HSQCsamplenot availablesample_conditions
CBCA(CO)NHsamplenot availablesample_conditions
HNCACBsamplenot availablesample_conditions
HNCOsamplenot availablesample_conditions
HNCACOsamplenot availablesample_conditions
(H)CCONH-TOCSYsamplenot availablesample_conditions
H(C)CONH-TOCSYsamplenot availablesample_conditions
HBHA(CBCA)NHsamplenot availablesample_conditions
Me-CC-TOCSYsamplenot availablesample_conditions
Me-HCC-TOCSYsamplenot availablesample_conditions
HCCH-TOCSYsamplenot availablesample_conditions
HBCBCGCDHDsamplenot availablesample_conditions
HBCBCGCDCEHEsamplenot availablesample_conditions
15N-NOESYsamplenot availablesample_conditions
13C-NOESYsamplenot availablesample_conditions

Software:

AZARA v2, Wayne Boucher - processing

ANSIG v3.3, Per Kraulis - assignment

formatConverter v1.0.b18, CCPN/EMBL - conversion to NMR_STAR

NMR spectrometers:

  • Bruker Avance 600.13 MHz
  • Bruker Avance 599.98 MHz
  • Bruker Avance 800.13 MHz
  • Bruker Avance 700.13 MHz
  • Varian Inova 600.13 MHz

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