BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6365

Title: 1H, 13C 15N chemical shift assignment of B. cereus protein BC4709, Northeast Structural Genomics Target Protein BcR68   PubMed: 16027363

Deposition date: 2004-10-27 Original release date: 2008-08-28

Authors: Liu, Gaohua; Acton, Thomas; Parish, David; Ma, Lichung; Xu, Duanxiang; Xiao, Rong; Montelione, Gaetano; Szyperski, Thomas

Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination"  Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).

Assembly members:
Protein BC4709, polymer, 143 residues, Formula weight is not available

Natural source:   Common Name: Bacillus cereus   Taxonomy ID: 1396   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Protein BC4709: MEQQNTLNDIKQTIVFNASI QKVWSVVSTAEGIASWFMPN DFVLEVGHEFHVQSPFGPSP CKVLEIDEPNHLSFSWDTDG WVVSFDLKDLGDNKTEFTLI HGGWKHPDEILPKANAKSSI IRDRMSGGWVAIVNEKLKKV VEG

Data sets:
Data typeCount
1H chemical shifts1026
13C chemical shifts513
15N chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein BC47091

Entities:

Entity 1, Protein BC4709 143 residues - Formula weight is not available

1   METGLUGLNGLNASNTHRLEUASNASPILE
2   LYSGLNTHRILEVALPHEASNALASERILE
3   GLNLYSVALTRPSERVALVALSERTHRALA
4   GLUGLYILEALASERTRPPHEMETPROASN
5   ASPPHEVALLEUGLUVALGLYHISGLUPHE
6   HISVALGLNSERPROPHEGLYPROSERPRO
7   CYSLYSVALLEUGLUILEASPGLUPROASN
8   HISLEUSERPHESERTRPASPTHRASPGLY
9   TRPVALVALSERPHEASPLEULYSASPLEU
10   GLYASPASNLYSTHRGLUPHETHRLEUILE
11   HISGLYGLYTRPLYSHISPROASPGLUILE
12   LEUPROLYSALAASNALALYSSERSERILE
13   ILEARGASPARGMETSERGLYGLYTRPVAL
14   ALAILEVALASNGLULYSLEULYSLYSVAL
15   VALGLUGLY

Samples:

sample_1: Protein BC4709, [U-13C; U-15N], 1.5 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 6.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • VARIAN Inova 750 MHz
  • VARIAN Inova 600 MHz

Related Database Links:

PDB
DBJ GAF00314
EMBL CCW07414 CDN38272
GB AAP11614 AAP28644 AAS43754 AAT34085 AAT56903
REF NP_834413 NP_847158 NP_981146 WP_000437627 WP_000437669

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts