BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6376

Title: Complete resonance assignment of the C-terminal domain from TgMIC1

Authors: Saouros, Savvas; Chen, Ho An; Cota, Ernesto; Edwards-Jones, Bryn; Simpson, Peter; Soldati-Favre, Dominique; Matthews, Stephen

Citation: Saouros, Savvas; Chen, Ho An; Simpson, Peter; Cota, Ernesto; Edwards-Jones, Bryn; Soldati-Favre, Dominique; Matthews, Stephen. "Letter to the Editor: Complete resonance assignments of the C-terminal domain from MIC1: A micronemal protein from Toxoplasma gondii"  J. Biomol. NMR 31, 177-178 (2005).

Assembly members:
TgMIC1-CT, polymer, 137 residues, 14020 Da.

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
TgMIC1-CT: KTEIHGDSTKATLEEGQQLT LTFISTKLDVAVGSCHSLVA NFLDGFLKFQTGSNSAFDVV EVEEPAGPAVLTIGLGHKGR LAVVLDYTRLNAALGSAAYV VEDSGCSSSEEVSFQGVGSG ATLVVTTLGESPTAVSA

Data sets:
Data typeCount
13C chemical shifts550
15N chemical shifts131
1H chemical shifts825

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TgMIC1-CT1

Entities:

Entity 1, TgMIC1-CT 137 residues - 14020 Da.

1   LYSTHRGLUILEHISGLYASPSERTHRLYS
2   ALATHRLEUGLUGLUGLYGLNGLNLEUTHR
3   LEUTHRPHEILESERTHRLYSLEUASPVAL
4   ALAVALGLYSERCYSHISSERLEUVALALA
5   ASNPHELEUASPGLYPHELEULYSPHEGLN
6   THRGLYSERASNSERALAPHEASPVALVAL
7   GLUVALGLUGLUPROALAGLYPROALAVAL
8   LEUTHRILEGLYLEUGLYHISLYSGLYARG
9   LEUALAVALVALLEUASPTYRTHRARGLEU
10   ASNALAALALEUGLYSERALAALATYRVAL
11   VALGLUASPSERGLYCYSSERSERSERGLU
12   GLUVALSERPHEGLNGLYVALGLYSERGLY
13   ALATHRLEUVALVALTHRTHRLEUGLYGLU
14   SERPROTHRALAVALSERALA

Samples:

sample_1: TgMIC1-CT, [U-95% 13C; U-95% 15N], 0.5 mM; Sodium Chloride 150 mM; sodium phosphate 20 mM

conditions_1: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
(H)CC(CO)NH TOCSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available

Software:

NMRView v4.1.3 -

XWIN-NMR -

NMRPipe -

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15685 15692
PDB
EMBL CAA96466
GB EEB01391 EEE22493 EEE31467
REF XP_002368531
SP O00834