BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6377

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for feline prion protein   PubMed: 15647367

Authors: Lysek, Dominikus; Nivon, Lucas; Wuthrich, Kurt

Citation: Lysek, Dominikus; Schorn, Christian; Nivon, Lucas; Esteve-Moya, Vicent; Christen, Barbara; Calzolai, Luigi; von Schroetter, Christine; Fiorito, Francesco; Herrmann, Torsten; Guntert, Peter; Wuthrich, Kurt. "Prion protein NMR structures of cats, dogs, pigs, and sheep"  Proc. Natl. Acad. Sci. U.S.A. 102, 640-645 (2005).

Assembly members:
fPrP, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: cat   Taxonomy ID: 9685   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Felis catus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
fPrP: GSVVGGLGGYMLGSAMSRPL IHFGNDYEDRYYRENMYRYP NQVYYRPVDQYSNQNNFVHD CVNITVRQHTVTTTTKGENF TETDMKIMERVVEQMCVTQY QKESEAYYQRRAS

Data sets:
Data typeCount
13C chemical shifts342
15N chemical shifts126
1H chemical shifts762

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fPrP monomer1

Entities:

Entity 1, fPrP monomer 113 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYASNASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLN
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRVALARGGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPMETLYSILEMETGLUARG
10   VALVALGLUGLNMETCYSVALTHRGLNTYR
11   GLNLYSGLUSERGLUALATYRTYRGLNARG
12   ARGALASER

Samples:

sample_1: fPrP, [U-13C; U-15N], 1 mM; sodium acidate 10 mM

conditions_1: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
13C edited noesysample_1not availableconditions_1
15N edited noesysample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker DRX 500 MHz
  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
GB AAS94127 ABY83003 ACA50722 ACA50727 ACA50730
SP O18754