BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6380

Title: 1H, 13C, and 15N Chemical Shift Assignments for pig prion protein   PubMed: 15647367

Authors: Schorn, Christian; Lysek, Dominikus; Wuthrich, Kurt

Citation: Lysek, Dominikus; Schorn, Christian; Nivon, Lucas; Esteve-Moya, Vicent; Christen, Barbara; Calzolai, Luigi; von Schroetter, Christine; Fiorito, Francesco; Herrmann, Torsten; Guntert, Peter; Wuthrich, Kurt. "Prion protein NMR structures of cats, dogs, pigs, and sheep"  Proc. Natl. Acad. Sci. U.S.A. 102, 640-645 (2005).

Assembly members:
pig prion protein, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: Pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Entity Sequences (FASTA):
pig prion protein: GSVVGGLGGYMLGSAMSRPL IHFGSDYEDRYYRENMYRYP NQVYYRPVDQYSNQNSFVHD CVNITVKQHTVTTTTKGENF TETDVKMIERVVEQMCITQY QKEYEAYAQRGAS

Data sets:
Data typeCount
13C chemical shifts307
15N chemical shifts123
1H chemical shifts716

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1pig prion protein monomer1

Entities:

Entity 1, pig prion protein monomer 113 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYSERASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLN
6   TYRSERASNGLNASNSERPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETILEGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNLYSGLUTYRGLUALATYRALAGLNARG
12   GLYALASER

Samples:

sample_1: pig prion protein, [U-13C; U-15N], 1 mM

conditions_1: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
15N edited NOESYsample_1not availableconditions_1
13C edited NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

PDB
GB AAA92862 AAV83492 AAV83493 AAV83494 AAV83495
REF NP_001008687
SP P49927