BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6381

Title: NMR solution structures of mammalian prion proteins   PubMed: 15647367

Authors: Calzolai, Luigi; Pioda, Giogio; Lysek, Dominikus

Citation: Lysek, Dominikus; Schorn, Christian; Nivon, Lucas; Esteve-Moya, Vicent; Christen, Barbara; Calzolai, Luigi; von Schroetter, Christine; Fiorito, Francesco; Herrmann, Torsten; Guntert, Peter; Wuthrich, Kurt. "Prion protein NMR structures of cats, dogs, pigs, and sheep"  Proc. Natl. Acad. Sci. U.S.A. 102, 640-645 (2005).

Assembly members:
sheep prion protein, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: Sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Entity Sequences (FASTA):
sheep prion protein: GSVVGGLGGYMLGSAMSRPL IHFGNDYEDRYYRENMYRYP NQVYYRPVDHYSNQNNFVHD CVNITVKQHTVTTTTKGENF TETDIKIMERVVEQMCITQY QRESQAYYQRGAS

Data sets:
Data typeCount
13C chemical shifts360
15N chemical shifts130
1H chemical shifts801

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ovPrP,H168 monomer1

Entities:

Entity 1, ovPrP,H168 monomer 113 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYASNASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPHIS
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPILELYSILEMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNARGGLUSERGLNALATYRTYRGLNARG
12   GLYALASER

Samples:

sample_1: sheep prion protein, [U-13C; U-15N], 1.0 mM

conditions_1: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
15N edited 1H1H NOESYsample_1not availableconditions_1
13C edited 1H1H NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

BMRB 6403
PDB
DBJ BAA07376 BAB69955 BAB69956 BAB69957 BAH03441
EMBL CAA04235 CAA04236 CAA04272 CAA04273 CAA04274
GB AAB70468 AAB97765 AAC78726 AAD14409 AAD19980
PRF 1921132A
REF NP_001009481
SP P23907 P52113 Q5UJG1 Q5XVM4 Q68G95