BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6382

Title: 1H, 13C, and 15N Chemical Shift Assignments for Xenopus prion protein.

Authors: Perez, Daniel; Wuthrich, Kurt

Citation: Perez, Daniel; Wuthrich, Kurt. "NMR assignment of the Xenopus leavis prion protein fragment xlPrP(98-226)"  J. Biomol. NMR 31, 260-260 (2005).

Assembly members:
prion, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus leavis

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Entity Sequences (FASTA):
prion: GSGYNKQWKPPKSKTNMKSV AIGAAAGAIGGYMLGNAVGR MSYQFNNPMESRYYNDYYNQ MPNRVYRPMYRGEEYVSEDR FVRDCYNMSVTEYIIKPAEG KNNSELNQLDTTVKSQIIRE MCITEYRRGS

Data sets:
Data typeCount
13C chemical shifts416
15N chemical shifts150
1H chemical shifts908

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1prion protein1

Entities:

Entity 1, prion protein 130 residues - Formula weight is not available

1   GLYSERGLYTYRASNLYSGLNTRPLYSPRO
2   PROLYSSERLYSTHRASNMETLYSSERVAL
3   ALAILEGLYALAALAALAGLYALAILEGLY
4   GLYTYRMETLEUGLYASNALAVALGLYARG
5   METSERTYRGLNPHEASNASNPROMETGLU
6   SERARGTYRTYRASNASPTYRTYRASNGLN
7   METPROASNARGVALTYRARGPROMETTYR
8   ARGGLYGLUGLUTYRVALSERGLUASPARG
9   PHEVALARGASPCYSTYRASNMETSERVAL
10   THRGLUTYRILEILELYSPROALAGLUGLY
11   LYSASNASNSERGLULEUASNGLNLEUASP
12   THRTHRVALLYSSERGLNILEILEARGGLU
13   METCYSILETHRGLUTYRARGARGGLYSER

Samples:

sample_1: prion, [U-13C; U-15N], 1 mM

conditions_1: pH: 4.5; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HCcH TOCSYsample_1not availableconditions_1
HCcH COSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAC86159
GB AAH94089 AAV54126
REF NP_001082180