BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6383

Title: NMR solution structure of the recombinant elk and mouse/elk variant prion proteins

Authors: Gossert, Alvar; Bonjour, Sophie; Lysek, Dominikus

Citation: Gossert, Alvar; Bonjour, Sophie; Lysek, Dominikus; Fiorito, Francesco; Wuthrich, Kurt. "Prion protein NMR structures of the elk and of mouse/elk hybrids"  Proc. Natl. Acad. Sci. U.S.A. 102, 646-650 (2005).

Assembly members:
elk prion protein, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: American elk   Taxonomy ID: 9864   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: cervus Cervus elaphus

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Entity Sequences (FASTA):
elk prion protein: GSVVGGLGGYMLGSAMSRPL IHFGNDYEDRYYRENMYRYP NQVYYRPVDQYNNQNTFVHD CVNITVKQHTVTTTTKGENF TETDIKMMERVVEQMCITQY QRESEAYYQRGAS

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts132
1H chemical shifts800

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1eprp monomer1

Entities:

Entity 1, eprp monomer 113 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYASNASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLN
6   TYRASNASNGLNASNTHRPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPILELYSMETMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNARGGLUSERGLUALATYRTYRGLNARG
12   GLYALASER

Samples:

sample_1: elk prion protein, [U-13C; U-15N], 1 mM

conditions_1: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
15N-edited NOESYsample_1not availableconditions_1
13C-edited NOESYsample_1not availableconditions_1
HNCAsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

PDB
EMBL CAA70902
GB AAA68941 AAB61573 AAB94788 AAC12860 AAC12861
SP P47852 P67986 P67987 Q5UJG3