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Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6387

Title: Structural and Functional Study of Anemonia Elastase Inhibitor, a "Non-classical" Kazal-type Inhibitor from Anemonia sulcata   PubMed: 16008348

Authors: Hemmi, Hikaru; Kumazaki, Takashi; Yoshizawa-Kumagaye, Kumiko; Nishiuchi, Yuji; Yoshida, Takuya; Ohkubo, Tadayasu; Kobayashi, Yuji

Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yoshizawa-Kumagaye, Kumiko; Nishiuchi, Yuji; Yoshida, Takuya; Ohkubo, Tadayasu; Kobayashi, Yuji. "Structural and Functional Study of an Anemonia Elastase Inhibitor, a "Nonclassical" Kazal-Type Inhibitor from Anemonia sulcata."  Biochemistry 44, 9626-9636 (2005).

Assembly members:
anemonia elastase inhibitor analogue, polymer, 48 residues, Formula weight is not available

Natural source:   Common Name: Anemonia sulcata   Taxonomy ID: 6108   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Anemonia sulcata

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
anemonia elastase inhibitor analogue: KPDAPCICTMQYDPVCGSDG ITYGNACMLLCASARSDTPI ELVHKGRC

Data sets:
Data typeCount
1H chemical shifts317

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1aei-analogue monomer1

Entities:

Entity 1, aei-analogue monomer 48 residues - Formula weight is not available

1   LYSPROASPALAPROCYSILECYSTHRMET
2   GLNTYRASPPROVALCYSGLYSERASPGLY
3   ILETHRTYRGLYASNALACYSMETLEULEU
4   CYSALASERALAARGSERASPTHRPROILE
5   GLULEUVALHISLYSGLYARGCYS

Samples:

sample_1: anemonia elastase inhibitor analogue3.0 – 3.5 mM

Ex-cond_1: pH: 2.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQFCOSYnot availablenot availablenot available
1H-1H ECOSYnot availablenot availablenot available
1H-1H ROESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

xwinnmr - processing

SPARKY - assignment, peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

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