BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6403

Title: Ovine Prion Protein Variant R168

Authors: Christen, Barbara; Wuethrich, Kurt

Citation: Lysek, Dominikus; Schorn, Christian; Nivon, Lucas; Esteve-Moya, Vicent; Christen, Barbara; Calzolai, Luigi; von Schoetter, Christine; Fiorito, Francesco; Herrmann, Torsten; Guntert, Peter; Wuthrich, Kurt. "Prion protein NMR structures of cat, dog, pig, and sheep"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:
sheep prion protein, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: Sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Entity Sequences (FASTA):
sheep prion protein: GSVVGGLGGYMLGSAMSRPL IHFGNDYEDRYYRENMYRYP NQVYYRPVDRYSNQNNFVHD CVNITVKQHTVTTTTKGENF TETDIKIMERVVEQMCITQY QRESQAYYQRGAS

Data sets:
Data typeCount
13C chemical shifts353
15N chemical shifts131
1H chemical shifts808

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ovine Prion Protein Variant R1681

Entities:

Entity 1, Ovine Prion Protein Variant R168 113 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYASNASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPARG
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPILELYSILEMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNARGGLUSERGLNALATYRTYRGLNARG
12   GLYALASER

Samples:

sample_1: sheep prion protein, [U-15N], 0.75 mM; sodium acetate, [U-2H], 10 mM; H2O 90%; D2O 10%

sample_2: sheep prion protein, [U-13C; U-15N], 0.5 mM; sodium acetate, [U-2H], 10 mM; D2O 100%

conditions_1: pH: 4.50; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availableconditions_1
3D 13C-separated NOESY (ali)not availablenot availableconditions_1
3D 13C-separated NOESY (arom)not availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker AVANCE 900 MHz

Related Database Links:

BMRB 6381
PDB
DBJ BAA07376 BAB69955 BAB69956 BAB69957 BAH03441
EMBL CAA04235 CAA04236 CAA04272 CAA04273 CAA04274
GB AAB70468 AAB97765 AAC78726 AAD14409 AAD19980
PRF 1921132A
REF NP_001009481
SP P23907 P52113 Q5UJG1 Q5XVM4 Q68G95