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Biological Magnetic Resonance Data Bank


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BMRB Entry 6407

Title: 1H, 15N resonance assignment of the C-terminal domain of Human Papillomavirus Oncoprotein E6

Authors: Nomine, Yves; Trave, Gilles; Kieffer, Bruno

Citation: Nomine, Yves; Charbonnier, Sebastian; Miguet, Laurent; Potier, Noelle; Van Dorsselaer, Alain; Atkinson, R.; Trave, Gilles; Kieffer, Bruno. "1H and 15N resonance assignment, secondary structure and dynamic behaviour of the C-terminal domain of human papillomavirus Oncoprotein E6"  J. Biomol. NMR 31, 129-141 (2005).

Assembly members:
C terminal domain of E6, polymer, 75 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Viruses   Kingdom: dsDNA viruses   Genus/species: Papillomavirus Human Papillomavirus type E6

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
C terminal domain of E6: GAMSYSLYGTTLEQQYNKPL SDLLIRCINCQKPLSPEEKQ RHLDKKQRFHNIRGRWTGRC MSCSRSSRTRRETQL

Data sets:
Data typeCount
15N chemical shifts78
1H chemical shifts513

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HPV16 E6 C-terminal domain1
2ZINC (II) ION2

Entities:

Entity 1, HPV16 E6 C-terminal domain 75 residues - Formula weight is not available

1   GLYALAMETSERTYRSERLEUTYRGLYTHR
2   THRLEUGLUGLNGLNTYRASNLYSPROLEU
3   SERASPLEULEUILEARGCYSILEASNCYS
4   GLNLYSPROLEUSERPROGLUGLULYSGLN
5   ARGHISLEUASPLYSLYSGLNARGPHEHIS
6   ASNILEARGGLYARGTRPTHRGLYARGCYS
7   METSERCYSSERARGSERSERARGTHRARG
8   ARGGLUTHRGLNLEU

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C terminal domain of E6, [U-15N], 0.3 – 1.2 mM; Tris-HCl deuterated 20 mM; NaCl 50 mM; dithiothreitol 1 mM; deuterium oxide 10%

conditions_1: pH: 6.8; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
15N NOESY-HSQCsample_1not availableconditions_1
15N TOCSY-HSQCsample_1not availableconditions_1

Software:

NMRPipe -

XEASY -

NMR spectrometers:

  • Bruker DMX 500 MHz

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