BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6419

Title: Structure of the C-terminal Domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2   PubMed: 15557330

Authors: Chattopadhyay, A.; Jones, N.; Nietlispach, D.; Nielsen, P.; Voorheis, H.; Mott, H.; Carrington, M.

Citation: Chattopadhyay, A.; Jones, N.; Nietlispach, D.; Nielsen, P.; Voorheis, H.; Mott, H.; Carrington, M.. "Structure of the C-terminal Domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2"  J. Biol. Chem. 280, 7228-7235 (2005).

Assembly members:
Variant surface glycoprotein MITAT 1.2, polymer, 80 residues, Formula weight is not available

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Variant surface glycoprotein MITAT 1.2: GSHMLEVLTQKHKPAESQQQ AAETEGSCNKKDQNECKSPC KWHNDAENKKCTLDKEEAKK VADETAKDGKTGNTNTTGSS

Data sets:
Data typeCount
13C chemical shifts231
15N chemical shifts79
1H chemical shifts433

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Variant surface glycoprotein MITAT 1.21

Entities:

Entity 1, Variant surface glycoprotein MITAT 1.2 80 residues - Formula weight is not available

1   GLYSERHISMETLEUGLUVALLEUTHRGLN
2   LYSHISLYSPROALAGLUSERGLNGLNGLN
3   ALAALAGLUTHRGLUGLYSERCYSASNLYS
4   LYSASPGLNASNGLUCYSLYSSERPROCYS
5   LYSTRPHISASNASPALAGLUASNLYSLYS
6   CYSTHRLEUASPLYSGLUGLUALALYSLYS
7   VALALAASPGLUTHRALALYSASPGLYLYS
8   THRGLYASNTHRASNTHRTHRGLYSERSER

Samples:

sample_1: Variant surface glycoprotein MITAT 1.2, [U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_2: Variant surface glycoprotein MITAT 1.2, [U-13C; U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-SEPARATED NOESYnot availablenot availablenot available
3D 13C-SEPARATED NOESYnot availablenot availablenot available
CLEANEXnot availablenot availablenot available

Software:

ARIA v1.1 - refinement, structure solution

AZARA v2.7 - structure solution

ANSIG v3.3 - structure solution

CNS v1.1 - structure solution

NMR spectrometers:

  • BRUKER DRX 600 MHz

Related Database Links:

PDB
EMBL CAA40081 CAQ57294
SWISS-PROT P26332