BMRB Entry 6441
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6441
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Title: Chemical shift assignments for human Orexin-A PubMed: 16429482
Deposition date: 2004-12-22 Original release date: 2006-04-17
Authors: Ikegami, Takahisa; Takai, Tomoyo
Citation: Takai, Tomoyo; Takaya, Takao; Nakano, Mutsuko; Akutsu, Hideo; Nakagawa, Atsushi; Aimoto, Saburo; Nagai, Katsuya; Ikegami, Takahisa. "Orexin-A is composed of a highly conserved C-terminal and a specific, hydrophilic N-terminal region, revealing the structural basis of specific recognition by the orexin-1 receptor." J. Pept. Sci. 12, 443-454 (2006).
Assembly members:
human Orexin-A, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
human Orexin-A: XPLPDCCRQKTCSCRLYELL
HGAGNHAAGILTX
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 54 |
15N chemical shifts | 31 |
1H chemical shifts | 181 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | human Orexin-A | 1 |
Entities:
Entity 1, human Orexin-A 33 residues - Formula weight is not available
1 | PCA | PRO | LEU | PRO | ASP | CYS | CYS | ARG | GLN | LYS | ||||
2 | THR | CYS | SER | CYS | ARG | LEU | TYR | GLU | LEU | LEU | ||||
3 | HIS | GLY | ALA | GLY | ASN | HIS | ALA | ALA | GLY | ILE | ||||
4 | LEU | THR | NLW |
Samples:
sample_1: human Orexin-A 0.76 mM
conditions_1: ionic strength: 18 mM; pH: 6.0; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D TOCSY | sample_1 | not available | conditions_1 |
2D NOESY | sample_1 | not available | conditions_1 |
DQF-COSY | sample_1 | not available | conditions_1 |
15N-1H-HSQC | sample_1 | not available | conditions_1 |
13C-1H-HSQC | sample_1 | not available | conditions_1 |
Software:
Sparky - chemical shift assignment
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts