BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6447

Title: Solution structure of a two-repeat fragment of major vault protein   PubMed: 16373071

Authors: Kozlov, G.; Vavelyuk, O.; Minailiuc, O.; Banville, D.; Gehring, K.; Ekiel, I.

Citation: Kozlov, G.; Vavelyuk, O.; Minailiuc, O.; Banville, D.; Gehring, K.; Ekiel, I.. "Solution Structure of a Two-repeat Fragment of Major Vault Protein."  J. Mol. Biol. 356, 444-452 (2006).

Assembly members:
Major vault protein, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Major vault protein: GSHMQVVLPNTALHLKALLD FEDKDGDKVVAGDEWLFEGP GTYIPRKEVEVVEIIQATII RQNQALRLRARKECWDRDGK ERVTGEEWLVTTVGAYLPAV FEEVLDLVDAVIL

Data sets:
Data typeCount
13C chemical shifts204
15N chemical shifts110
1H chemical shifts642

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mv_protein1

Entities:

Entity 1, mv_protein 113 residues - Formula weight is not available

1   GLYSERHISMETGLNVALVALLEUPROASN
2   THRALALEUHISLEULYSALALEULEUASP
3   PHEGLUASPLYSASPGLYASPLYSVALVAL
4   ALAGLYASPGLUTRPLEUPHEGLUGLYPRO
5   GLYTHRTYRILEPROARGLYSGLUVALGLU
6   VALVALGLUILEILEGLNALATHRILEILE
7   ARGGLNASNGLNALALEUARGLEUARGALA
8   ARGLYSGLUCYSTRPASPARGASPGLYLYS
9   GLUARGVALTHRGLYGLUGLUTRPLEUVAL
10   THRTHRVALGLYALATYRLEUPROALAVAL
11   PHEGLUGLUVALLEUASPLEUVALASPALA
12   VALILELEU

Samples:

sample_1: Major vault protein, [U-15N], 1 mM; bis-Tris 20 mM; NaCl 150 mM; CaCl2 2 mM; DTT 15 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

xwinnmr v2.1 - collection, processing

XEASY v1.3.13 - data analysis

CYANA v1.0.6 - structure solution

X-PLOR NIH v2.9.2 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AHW56615