BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6459

Title: Chemical Shift Assignment for Itch E3 WW3 domain   PubMed: 15971201

Authors: Shaw, Alison; Martin-Malpartida, Pau; Morales, Begonya; Yraola, Francesc; Royo, Miriam; Macias, Maria

Citation: Shaw, Alison; Martin-Malpartida, Pau; Morales, Begonya; Yraola, Francesc; Royo, Miriam; Macias, Maria. "Phosphorylation of either Ser16 or Thr30 does not disrupt the structure of the Itch E3 ubiquitin ligase third WW domain"  Proteins 60, 558-560 (2005).

Assembly members:
WW 3 domain, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
WW 3 domain: GAMGPLPPGWEKRTDSNGRV YFVNHNTRITQWEDPRS

Data sets:
Data typeCount
13C chemical shifts118
15N chemical shifts42
1H chemical shifts386

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WW 3 domain1

Entities:

Entity 1, WW 3 domain 37 residues - Formula weight is not available

1   GLYALAMETGLYPROLEUPROPROGLYTRP
2   GLULYSARGTHRASPSERASNGLYARGVAL
3   TYRPHEVALASNHISASNTHRARGILETHR
4   GLNTRPGLUASPPROARGSER

Samples:

unlabeled: WW 3 domain 1 mM; NaCl 100 mM; Na2HPO4 50 mM

15N_labeled: WW 3 domain, [U-15N], 1 mM; NaCl 100 mM; Na2HPO4 50 mM

13C_15N_labeled: WW 3 domain, [U-13C; U-15N], 1 mM; NaCl 100 mM; Na2HPO4 50 mM

conditions_1: pH: 6.0; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
1H15N-HSQC15N_labelednot availableconditions_1
CBCA(CO)NH13C_15N_labelednot availableconditions_1
CBCANH13C_15N_labelednot availableconditions_1
15N_edited_TOCSY15N_labelednot availableconditions_1
15N_edited_NOESY15N_labelednot availableconditions_1
13C_edited_NOESY13C_15N_labelednot availableconditions_1
13C_edited_TOCSY13C_15N_labelednot availableconditions_1
(H)CC(CO)NH13C_15N_labelednot availableconditions_1
H(CC)(CO)NH13C_15N_labelednot availableconditions_1

Software:

CNS, Bruenger AT - structure calculation

xwinnmr, Bruker Biospin - acquisition

XEASY, Bartels, C. - assignment

NMRPipe, Delaglio, F. - processing

ARIA, Nilges, M. - assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15153 15159
PDB