BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6465

Title: 1H, 13C, and 15N chemical shift assignments for PAL

Authors: Parsons, Lisa; Orban, John

Citation: Parsons, Lisa; Orban, John. "NMR assignment of the periplasmic domain of peptidoglycan-associated lipoprotein (Pal) from Haemophilus influenzae"  J. Biomol. NMR 32, 93-93 (2005).

Assembly members:
PAL - Peptidoglycan associated lipoprotein, polymer, 134 residues, Formula weight is not available
UDP-MurNAc-pentapeptide(UYP), polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: H. influenzae   Taxonomy ID: 727   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PAL - Peptidoglycan associated lipoprotein: CSSSNNDAAGNGAAQTFGGY SVADLQQRYNTVYFGFDKYD ITGEYVQILDAHAAYLNATP AAKVLVEGNTDERGTPEYNI ALGQRRADAVKGYLAGKGVD AGKLGTVSYGEEKPAVLGHD EAAYSKNRRAVLAY
UDP-MurNAc-pentapeptide(UYP): XXAXXXX

Data sets:
Data typeCount
13C chemical shifts503
1H chemical shifts831
15N chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAL - Peptidoglycan associated lipoprotein1
2UDP-MurNAc-pentapeptide(UYP)2

Entities:

Entity 1, PAL - Peptidoglycan associated lipoprotein 134 residues - Formula weight is not available

1   CYSSERSERSERASNASNASPALAALAGLY
2   ASNGLYALAALAGLNTHRPHEGLYGLYTYR
3   SERVALALAASPLEUGLNGLNARGTYRASN
4   THRVALTYRPHEGLYPHEASPLYSTYRASP
5   ILETHRGLYGLUTYRVALGLNILELEUASP
6   ALAHISALAALATYRLEUASNALATHRPRO
7   ALAALALYSVALLEUVALGLUGLYASNTHR
8   ASPGLUARGGLYTHRPROGLUTYRASNILE
9   ALALEUGLYGLNARGARGALAASPALAVAL
10   LYSGLYTYRLEUALAGLYLYSGLYVALASP
11   ALAGLYLYSLEUGLYTHRVALSERTYRGLY
12   GLUGLULYSPROALAVALLEUGLYHISASP
13   GLUALAALATYRSERLYSASNARGARGALA
14   VALLEUALATYR

Entity 2, UDP-MurNAc-pentapeptide(UYP) 7 residues - Formula weight is not available

1   UDPAMUALADGNKCXDALDAL

Samples:

sample_1: PAL - Peptidoglycan associated lipoprotein, [U-13C; U-15N], 0.8 mM; UDP-MurNAc-pentapeptide(UYP), [U-13C; U-15N], 0.8 mM; sodium phospate 50 mM; sodium chloride 50 mM; EDTA 0.05 mM

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HCCONHsample_1not availableconditions_1
CCONHsample_1not availableconditions_1
aromatic constant-time 13C-HSQCsample_1not availableconditions_1
2DTOCSYsample_1not availableconditions_1
2DNOESYsample_1not availableconditions_1
13C-3D NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

REF ZP_00155384 YP_001292339 YP_248096 NP_438542 YP_001290090
SP P10324
GB AAX87436 ABQ97707 AAA24994 AAC22039 AAA24940
EMBL CBW28680
BMRB 6858
PDB