BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6476

Title: Full 1H, 13C and 15N Chemical Shift Assignments for the GII loop mutant domain (GII-L) of the yeast linker histone, Hho1p   PubMed: 15878177

Authors: Sanderson, Andrew; Stott, Katherine; Stevens, Timothy; Thomas, Jean

Citation: Sanderson, Andrew; Stott, Katherine; Stevens, Timothy; Thomas, Jean. "Engineering the structural stability and functional properties of the GI domain into the intrinsically unfolded GII domain of the yeast linker histone Hho1p."  J. Mol. Biol. 349, 608-620 (2005).

Assembly members:
GII-L, polymer, 87 residues, 9537 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GII-L: KASSPSSLTYKEMILKSMPQ LNDGKGSSRIVLKKYVKDTY PIVGSASNFDYLFNSAIKKC VENGELVQPKGPSGIIKLNK KKVKLST

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts88
1H chemical shifts636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GII-L1

Entities:

Entity 1, GII-L 87 residues - 9537 Da.

1   LYSALASERSERPROSERSERLEUTHRTYR
2   LYSGLUMETILELEULYSSERMETPROGLN
3   LEUASNASPGLYLYSGLYSERSERARGILE
4   VALLEULYSLYSTYRVALLYSASPTHRTYR
5   PROILEVALGLYSERALASERASNPHEASP
6   TYRLEUPHEASNSERALAILELYSLYSCYS
7   VALGLUASNGLYGLULEUVALGLNPROLYS
8   GLYPROSERGLYILEILELYSLEUASNLYS
9   LYSLYSVALLYSLEUSERTHR

Samples:

sample_1: GII-L, [U-13C; U-15N], 1 mM; sodium phosphate buffer 100 mM; EDTA 1 mM; DTT, [U-2H], 1 – 2 mM

conditions_1: pH: 7.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
(H)CC(CO)NH-TOCSYsample_1not availableconditions_1
TOCSY-15N-HSQCsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
NOESY-15N-HSQCsample_1not availableconditions_1
NOESY-13C-HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Burker DRX 500 MHz

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